The Effects Of Secreting Cytokines(IL-17/IFN-?) Of Different Phenotypic Th17 Cells On Regulating Primary Osteoblasts Proliferation,Differentiation And Calcification In Vitro

Objective:In order to investigate the effects of secreting cytokines(IL-17/IFN-?)of different phenotypic Th17 cells on regulating primary osteoblasts proliferation,differentiation and calcification,and clarify the synergistic or antagonistic effect on alveolar bone remodeling between IL-17 and IFN-y.Methods:To evaluate osteogenesis in vitro,primary mouse calvarial osteoblasts were extracted by enzymatically digesting calvarial bone from neonatal mouse and were treated by IL-17,IFN-y,IL-17+IFN-? based on induced medium.The proliferation of osteoblasts was evaluated by MTT assay at 24h,48h,72h and 96h.Osteogenic activity was tested by alkaline phosphatase(ALP)activity assay and alizarin red staining.The mRNA levels of ALP,osteocalcin,osteoprotegerin(OPG),Runt-related transcription factor 2(Runx2)and receptor activator of nuclear factor-kappa B ligand(RANKL)were also measured by real-time quantitative PCR.The JAK2-STAT3 pathway was evaluated by Western blot.Results:In our study,we found that primary osteoblasts were mononuclear,spindle-shape and polygonal.Osteoblasts showed no obvious proliferation when treated with IL-17 and/or IFN-y,but higher ALP activities were observed in primary osteoblasts treated with IL-17 or IL-17+IFN-y in induction medium.We also found that IL-17 could promote the gene expression of Alp,Runx2,Osteocalcin,Opg,and Rankl,while IFN-y might attenuate this effect.Nevertheless,IL-17 and IFN-y exhibited an inhibitory effect on the calcification of primary osteoblasts.We also found that IL-17 could directly facilitate RANKL expressions by JAK2-STAT3 pathway.Conclusions:The positive effects of different phenotypes of Thl 7 cells secreting cytokines(IL-17/IFN-y)on the early-stage differentiation and the negative effects on the calcification of murine calvarial osteoblasts contribute to our understanding of the role and interaction of inflammatory factors in the bone remodeling and as fundamental mechanisms involved in the destruction of alveolar bone.Objective:To observe clinical outcomes of concentrate growth factors on guided tissue regeneration for patients with chronic periodontitis,and to evaluate the therapeutical effect of combined GTR with CGF to periodontal tissue regeneration.Methods:3 cases of GTR operation were chosen,which involved CGF used in GTR treatment on the basis of basic periodontal therapy.During the whole treatment processes,regular periodontal maintenance was necessary,clinical periodontal tissue conditions were examined,such as PD,BOP and width of keratinized gingiva.Meanwhile,statuses of alveolar bone were evaluated before and after treatment with the application of X-rays.Results:Two weeks after surgery,the degree of mucosal healing was healing by first intention without hemorrhage and infection.After more than three months after-surgery follow-up,no more root hypersensitivity and other symptoms were complained.Partial sites gained wider keratinized gingiva and periodontal bone regeneration was promoted to some different extents.Conclusions:The combination of GTR and CGF can promote periodontal tissue regeneration to a certain extent.