| Objective: To evaluate the role of CGF(Concentrate growth factor,CGF)in the formation and improvement of soft tissue in anterior dental implant,and to provide basic information for further clinical application.Methods:1.Medical records:From November 2016 to October 2017,15 cases and20 site of anterior tooth implantation in the Department of Implant of Bethune International Peace Hospital were selected.CGF preparation method:Venous blood was collected,separate and standby for the operation.In put the implants then CGF was cut into particles and mixed with Bio-oss bone substitute,to fill the bone defect.And the CGF membrane was fixed and sutured to closed wound.The patients were examined for gingival soft tissue reconstruction 12 weeks after operation.The gingival tissue with a diameter of 2mm was cut off above the implant site,The specimen was made in the pathology department of our hospital;The ISQ(Implant stability Quotient)of the implant was measured.Patients with ISQ >60 were asked to make denture models 2 weeks later.The width of the keratinized gingival at the planting site was measured before surgery and at 4,12,14 and 16 weeks after surgery.The soft tissue thickness at the site was measured before and 12 weeks after surgery.Finally,using photoshop cs6 software to analyze and compare the color of keratinized gingival.The CGF sites of individual implants were evaluated for the aesthetic score PES of the tissue around the implants.2.Statistics and evaluation methods:The immediate implantation and early implantation on the increase of keratinized gingival was compared with the preoperative and postoperative 16 weeks of gingival growth.Comparing the keratinized gingival width and thegingival thickness before and after using CGF.The correlation analysis between PES score and postoperative gingival thickness of 10 single implants.The soft tissue color of the implant site was compared with contralateral homonym teeth on the RGB mean.Results:All cases were followed up to 6 months after operation.All implants were loaded after 16 weeks.The implant survival rate was 100%(20/20).1 Paired t-test statistical analysis was used to compare the different keratinized gingival growth between immediately planted and early implantation,P=0.23,P>0.05,there was no statistical significance.2 The effect of CGF on the width of keratinized gingival:2.1 Using One-way ANOVA of variance before operation,4 weeks after operation,postoperative 12 weeks,14 weeks after operation,16 weeks after operation(F=7.25,P=0.00,P<0.05)indicate that there were differences between the two groups before and after surgery,4,12,14 and 16,respectively.there was no statistical difference among the 4 groups after operation.2.2 Effects of CGF on gingival thickness:Preoperative gingival thickness,Gingival thickness 12 weeks after operation,There were statistical differences used paired t test(P=0.00,P<0.05).2.3 The effect of gingival thickness at the planting site on the PES score:The peason correlation analysis of 12 weeks thicknesses and their PES scores on 10 single-planting sites was performed.P=0.01,P<0.05,the results were reliable,and the correlation coefficient C=0.762 showed a significant correlation.3 keratinized gingiva texture evaluation3.1 RGB analysis results of gingival color:Two-sample paired t test was used to analyze the RGB in CGF group and the control group,P=0.73,P>0.05.There was no significant difference between the two groups.3.2 Planting site soft tissue specimen pathological section staining:The biopsy HE staining showed that the epidermis had a completekeratinized layer and the proliferation of squamous cells was obvious.Conclusion: CGF plays an active role in the quality and quantity of keratinized gingival around implants during the observation period in this study.It is speculated that CGF may be useful in reducing periimplant disease and aesthetic complications.The long-term effect of CGF on the soft tissue around the implant needs further study. |
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