Metabolic Remodeling Of Chronic Heart Failure By ~1H-NMR Metabonomics Clinical Research

Objective:The changes of serum metabolism group in patients with chronic heart failure were studied by using 1H-NMR metabolomics method,and serum biomarkers were screened to verify the feasibility of using this method in the diagnosis of chronic heart failure.On this basis,biomarkers with high correlation with chronic heart failure were screened,which provided an important diagnostic tool for the objective quantitative evaluation of myocardial metabolic remodeling in chronic heart failure,and provided clues for studying the metabolic mechanism of heart failure.Methods:The study included90 subjects,including 63 males and 27 females,with an average age of 62 years,including30 normal controls.60 patients with heart failure(including 30 patients with EF < 40%and 30 patients with 40% < EF < 50%)were asked to have no alcohol consumption within8 hours and 1 week before blood sampling.Coffee and other irritant drinks.The blood collected at room temperature naturally coagulated for 1 hour,collected upper serum 10000 turn / separation heart 10 min,collect upper serum,place jade 80 degrees Celsius refrigerator for cryopreservation.The testing instrument is model I of VARIAN,USA Nova600 MHz superconducting nuclear magnetic resonance spectrometer.The data were analyzed by principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DAA)by principal component analysis(SIMCA-P 11)and orthogonal partial least-squares discriminant analysis(OPLS-DAA),respectively.Serum metabolites were determined between CHF group and healthy control group.Comparing the discriminant ability of two pattern recognition methods.OPLS-DA Analyzer R2 X and Q2 parameters are used to evaluate the accuracy of the analysis results,where Q2 denotes the authenticity of the statistical results.In this study,we determined the different metabolites in each group by correlation coefficient correlation coefficient(r)obtained by OPLS-DA analysis.The test standard is p0.05.The critical value of Pearsonian productmoment correlation coefficient determined by Pearson correlation coefficient is equal to the significant difference level P0.05.The correlation coefficient r was determined as the threshold of significant difference in metabolite content,and the metabolite represented by r > threshold was a metabolite with statistically significant difference(P < 0.05).Results:There were significant differences in serum metabolites between EF < 40% contractile CHF group and healthy control group,but the other two groups had no significant difference.The differences of metabolites in each group were further compared by oneway-ANONA statistical method.The metabolites with no significant difference in concentrations among the three groups were excluded,and 3-Hydroxybutyratea,Acetoneum acetone,Alanine,valine,lactat lactic acid were screened out.There were significant differences among the five serum metabolites(P < 0.05),and acetone and 3-hydroxybutyric acid were positively correlated with NT-proBNP.Conclusion:1.The serum metabolites of EF < 40% contractile CHF group and healthy control group were significantly different,while 40% < EF < 50% contractile CHF group and control group had no significant difference in 1H-NMR profiles.2.All the serum metabolites,such as3-hydroxybutyric acid and acetone,were positively correlated with NT-proBNP.Therefore,we consider that acetone and 3-hydroxybutyric acid can provide some clinical diagnostic value in the diagnosis of contractile CHF.