The Study Of Sporothrix Schenckii Complex Interacting With Dendritic Cells To Produce IL-1 And IL-6

Objective: Sporotrichosis is a chronic infection caused by Sporothrix schenckii(S.schenckii)complex mucous membrane of the skin and subcutaneous tissue.In recent years,due to the prevalence of immunosuppressive agents and immunodeficiency diseases such as acquired immunodeficiency syndrome(AIDS),reports about the serious clinical types of the disease are increasing.Inherent immunity forms the frontier line of immune defense for antifungal infection of the body.Dendritic cells are the most powerful antigen presenting cells in the body.They are the primary effector cells of fungal immune response.The release of inflammatory factors can influence the development of immune inflammatory response after the DCs' been exposed to invasive pathogens.This paper will explore the IL-1? and IL-6 levels produced by DCs in the Sporothrix schenckii complex infection immune response,to reveal the possible role that dendritic cells played in the immune response of S.schenckii complex,and to provide a new target point for the treatment of Sporotrichosis.Methods:Use S.schenckii complex fungus suspension to infenct BMDCs,and use ELISA and Western-Blot to detect the secretion of IL-1? and IL-6.Results: S.schenckii complex can stimulate BMDC to secrete IL-1? and IL-6.The secretions of these two kinds of inflammatory factors are affected by the concentration of fungi and the duration of the stimulation.Conclusion: The levels of inflammatory factors secreted by DCs rely on the Pathogen concentration and the duration of the stimulation.It plays an important role in initiating and guiding the inflammatory response of the host.