Professor Zhu Jia's Experience In The Treatment Of Lung Cancer With Herb Pairs And The Mechanism Study Of Traditional Chinese Medicine On NSCLC Cells

Objective:To summarize the experience of tutor in the treatment of lung cancer and explore the mechanism of traditional Chinese medicine in the treatment of lung cancer.Methods:The first part summarizes the progress of traditional Chinese and western medicine research on lung cancer.In the second part,combinning with the modern research of the traditional Chinese medicine,summing up regular herb pairs of the tutor in treatment of lung cancer.In the third part,A549 of NSCLC cell lines were used.Cytotoxicity,in vitro tumorigenicity,gene expression,and protein expression were analyzed by MTT assay,soft agar assay,real-time PCR,and Western blots,respectively.Results:The article summarizes the common herbal pair to treat lung cancer by my tutor,including Astragalus membranaceus,Caulis Spatholobi and glossy privet fruit,asparagus fern and Radix Ophiopogonis,adenophora tetraphylla and radix glehniae,Morinda officinalis and herba epimedii which are used for strengthening the human body' s resistance;Rhizoma Pinellinae Praeparata and pericarpium citri reticulatae,almond and Thunberg Fritillary Bulb,grifola and Poria cocos,the root bark of the peony tree and the root of red-rooted salvia,herba lycopi and rhizoma alismatis which are used for eliminating phlegm,alleviating water retention and dispersing blood stasis;Sculellaria barbata and Oldenlandia diffusa,Chinese actinidia root and ternate buttercup root,edible tulip and raw oyster shell,Tianlong and lumbricus,scorpio and centipede which are used for anticancer,detoxifying and removing stasis;Platycodon grandiflorum and fructus aurantii,radix bupleuri and radix paeoniae alba which are used for regulating the flow of vital energy and removing obstruction toit,and dispelling melancholy;scorched hawthorn fruit and medicated leaven,fried rice sprout and roasted malt which are used for appetizing.The experimental research is as follows:The IC50 value of LJSJF and the monarch drug Sculellaria barbata on A549 cell was 3.5 mg/mL and 0.5 mg/mL respectively,and A<AB=AC<ABC<ABCD.A549 cell which was intervened by 0.2 mg/mL and 0.4 mg/mL Sculellaria barbata had apparent effect in aspects of size and quantity compared with the control group,and the same effect occurred for the group treated by 4mg/mL LJSJF.qRT-PCR analysis showed that the mTOR mRNA expression level in A549 cell which was intervened by 0.2 mg/mL and 0.4 mg/mL Sculellaria barbata reduced by 1.4 times and 2.5 times respectively,p38MAPK mRNA expression quantity reduced by 1.6 times and 2.1 times respectively,and EGFR mRNA expression quantity reduced by 2.7 times for the group intervened by 0.4mg/mL Sculellaria barbata.And after A549 cell was intervened by 2mg/mL and 4mg/mL LJSJF,the EGFR gene level reduced by 1.3 times and 2.3 times respectively,mTOR mRNA reduced by 1.3 times and 2.9 times respectively,p38MAPK reduced by 2.4 times and 2.2 times respectively,p21 gene level increased,while survivin declined;after being intervened by 0.2 mg/mL and 0.4 mg/mL Sculellaria barbata,p21 gene level increased by 14.4 times and 19.9 times respectively,survivin protein gene expression apparently reduced by 2.2 times and 3.3 times respectively,MDM2 gene expression apparently reduced by 1.3 times and 1.9 times respectively,with apparent dosage-dependence effect;additionally,after LJSJF was used for treatment,p53 protein expression significantly increased,gene expression increased by 1.5 times.Conclusion:There are a lot of herbs pairs in the treatment of lung cancer by tutor.LJSJF exerts more pronounced cytotoxic effects against NSCLC cells than SB does by synergistically regulating the underlining molecular mechanisms including EGFR and/or p53 signaling pathways.