Characteristics Of Intestinal Microflora In Patients With Coronary Heart Disease In Yunnan

Objective: The myriad of microbes distributed on human skin and mucosal surfaces and in the intestine are composed of bacteria,archaea,viruses,fungi,and other protozoa,collectively referred to as microbiota.About 1,000 kinds of microorganisms settle in the human intestinal tract.Bacteria tend to be interconnected and symbiotic or parasitic.In all industrialized countries,the incidence and mortality of ischemic cardiomyopathy remain high.In recent years,China has seen a significant upward trend,with at least 500,000 new infections each year and at least 2 million patients.Many patients are experiencing persistent illness and eventually develop heart failure and even death.Studies at home and abroad have confirmed that phytide choline,a metabolite of intestinal flora,can promote cardiovascular disease.At the same time,phosphatidylcholine derivative trimethylamine(TMA)can cause atherosclerosis and promote the occurrence of cardiovascular disease.The study of the diversity,structure,and abundance of intestinal flora in patients with coronary heart disease can confirm whether the intestinal flora of patients with coronary heart disease has changed.It is envisaged that the abundance and distribution of gut flora in patients with coronary heart disease obtained from the study will be used as a control and compared with the gut microbiota of hospitalized patients.The higher similarity may indicate that the risk of coronary heart disease is higher.It can be used as a biomarker for the next step,and provides a theoretical basis for further treatment,providing reference for clinical application.Methods: According to the 2016 WHO diagnostic criteria,selected 67 patients with acute myocardial infarction,91 patients with coronary heart disease with unstable angina,47 patients with stable coronary heart disease,37 patients with coronary atherosclerosis,and 64 patients without coronary artery disease from Yunnan.The faeces DNA was extracted and 16 S r RNA v3 and v4 sequence-specific primers were used to perform real-time fluorescence quantitative PCR and Illumina sequencing.The diversity,structure,and abundance of the five groups of bacteria were compared.Results: Intestinal flora comparison 1.Comparison of bacterial classification in five groups of people There was no significant difference in the number of effective sequences in the five groups.There were no significant differences among the five groups in the classification of gates,classes,orders,families,and genera.2.Percentage of total bacteria in the five populations The thick-leaf bacillus was the most abundant in reading among the five groups,and the Bacteroides genus had the second highest reading abundance.Compared with the control group(group D),there was an increase in Fusobacterium in patients with acute MI(group A).The number of Bacteroides in patients with coronary heart disease and unstable angina(Group B)decreased,and Proteus and Actinomycetes increased.Coronary heart disease patients with stable angina(group C)decreased Bacteroides,Proteus,Fusobacterium increased.Patients with coronary atherosclerosis(Group E)have increased Proteus.Conclusion: The diversity,structure,and abundance of intestinal microbiota in the acute myocardial infarction group,the coronary heart disease unstable angina group,the coronary heart disease stable angina group,the coronary atherosclerosis group,and the control group were different.Patients with acute myocardial infarction,unstable angina pectoris of coronary heart disease,stable angina pectoris of coronary heart disease,and coronary atherosclerosis have different degrees of dysbacteriosis in the intestinal tract.