Analysis Of Treg Cell-mediated Immunity Imbanlance Mechanism And Syndromes Characteristics In Hepatitis C Cirrhosis Patients With HP Infection

Objective:To observe the effect of HP infection on liver function,investigate the mechanism of action for HP infection by Treg cells in hepatitis C cirrhosis patients and preliminarily understand these patients with TCM syndromes distribution characteristics,which lays the foundation for follow-up Chinese medicine interventional treatment.Methods:.The research is based on the patients in the first affiliated hospital of Henan University of TCM.1:Hepatitis C cirrhosis patients with HP infection are selected as the observation group while the others without HP are selected as the control group.Meanwhile,gene expression of the key factor“FoxP3”of peripheral blood Treg and ROR?t of Th17cells are detected by Real-time PCR and Elisa is used to test the peripheral blood~'s cytokines,such as IL-2?IL-6?IL-10?IL-17?IL-12?IFN-??TNF-?and TGF-?1.Investigators will record the general information and liver function data(ALT,AST,GGT and ALP),statistically compare the difference of two research groups and make a correlation analysis between the gene expression level of FoxP3 and cytokines indicators.2.A retrospective study will be conducted on patients with hepatitis C cirrhosis with or without HP infection over the past four years in the first affiliated hospital and compare their TCM syndrome constituent ratioResults:1.Liver damage degree of the observation group is significantly higher than the control group(P<0.05).2.The relative gene expression level of FoxP3 in peripheral blood of the observation group are higher than the control group and the differences are statistically significant(P<0.05).3.The expression level of IL-10?IL-6?IL-17?TGF-?1 in peripheral blood of the observation group are higher than the control group while the expression level of IL-2?IL-12?IFN-?are lower.The differences of them are statistically significant(P<0.05).the level of TNF-?in peripheral blood has no statistically significant difference(P>0.05).4.The gene expression of FoxP3 has the positive correlation with the expression level of IL-10?TGF-?1 in these two groups.It has no obvious correlation with the expression level of IL-12?IFN-??IL-6?TNF-?in observation group and with the expression level of IL-17?IL-12?IL-6?TNF-?in control group.In addition,it has the negative correlation with the expression level of IL-2?IL-17 in observation group and with the expression level of IL-2?IFN-?in control group.5.Syndrome research shows that the distribution of syndromes in Hepatitis C cirrhosis patients with HP infection group are as follows:Damp heat>Liver qi stagnation>Blood stasis>Wet resistance card>Liver and kidney yin deficiency>Spleen and kidney yang deficiency while in hepatitis C cirrhosis without HP infection group are as follows:Liver qi stagnation syndrome>Blood stasis syndrome>Damp heat syndrome>Wet resistance card>Liver and kidney yin deficiency>Spleen and kidney yang deficiency.The syndromes distributions of the two groups are significant difference.(P<0.05).Conclusion:1.HP infection will aggravate liver damage degree for hepatitis C cirrhosis patients.2.The liver damage degree of hepatitis C cirrhosis patients with HP infection may be correlated with high expression of Treg cells.3.Treg cells induce the mechanism of liver damage may be correlated with that they up-regulate the expression of IL-10,TGF-?1,IL-6 and IL-17 in peripheral blood,and down-regulate the expression of IL-2,IL-12 and IFN-?.4.The characteristics of syndromes for hepatitis C cirrhosis patients with HP infection indicate that HP may be"Damp heat"pathogenic factor to aggravate the hepatitis C disease,which provides an objective basis for the treatment of“Invigorating spleen,Qingre,LIshi”in hepatitis C related diseases.