Objective To Investigate The Effects Of Calcium Dibutyryladenosine Cyclophosphate On Hepatic Fibrosis Induced By Carbon Tetrachloride In Rats

Objective:The purpose of this study is to use animal experiments,observe Calcium Dibutyryladenosine Cyclophosphate on hepatic fibrosis of rats and pathological changes of liver tissue MMP-1,TIMP-1 expression,to study the effect of Calcium Dibutyryladenosine Cyclophosphate on liver fibrosis and intervention treatment,discusses the action mechanism,develop ideas for anti-fibrosis treatment.Methods:We were randomly divided into 3 groups,including:control group,model group,treatment group.In addition to the control group,the rats in model group and treatment group were injected intraperitoneal in experiment 40%CC1₄ olive oil mixed solution 1ml/kg on first day,thereafter every three days injected intraperitoneal in experiment 40%CC1₄ olive oil mixed solution 2ml/kg.The control group,intraperitoneal injection of the same amount of Sodium Chloride Physiological Solution injection every 3 days thereafter.Experimental sixth and seventh weekend the week randomly sacrificed model rats,light microscopy of liver pathological changes in the eighth weekend of the experiment successful modeling.After the modeling,given to treatment group rats was treated with Calcium Dibutyryladenosine Cyclophosphate?40mg/?Kg·d??of gavage.The model group tats gavage with saline.Number of dead rats in the control group,model group and treatment group were 1,3 and 2.At 12 weeks of the experiment,rats were fasted for12 hour.In each group,8 rats were randomly selected for deep anesthesia with ether.Liver tissue samples were collected in each group.HE staining and Masson staining were used to observe the pathological changes of liver tissues of rats in each group and semi-quantitative analysis of liver fibrosis was performed by Masson staining.Immunohistochemical staining and Western blotting were used to detect the expression of matrix metalloproteinase MMP-1 and its inhibitor TIMP-1 in the liver tissues of each group.Results:1.Liver tissue HE staining of the inflammatory activity score results:The model groupwas significantly higher than that in the normal group,which was significantly higher than that in the control group?P<0.01?.The model group(was significantly higher than that in the treatment group,which was significantly higher than that in the control group?P<0.01?.2.Liver tissue Masson staining of liver fibrosis score results:The model groupwas significantly higher than that in the normal group,which was significantly higher than that in the control group The model group was significantly higher than that in the treatment group,which was significantly higher than that in the control group?P<0.01?.3.Immunohistochemical staining of liver tissue:The expression level of MMP-1 in model group was significantly lower than that of control group?P<0.01?.The treatment group was significantly higher than the model group?P<0.01?.The expression level of TIMP-1 in control group was significantly lower than that of model group?P<0.01?,The model group was significantly higher than the treatment group?P<0.01?.4.Western blotting results show:The expression of MMP-1 protein in the control group was significantly higher than that in the model group?P<0.01?.The expression of MMP-1 protein in liver tissue of treatment group was significantly higher than that of model group?P<0.01?.The expression of TIMP-1 protein in the model group was significantly higher than that in the control group?P<0.01?.The expression of TIMP-1 protein in liver tissue of model group was significantly higher than that of treatment group?P<0.01?.Conclusions:Calcium Dibutyryladenosine Cyclophosphate can reduce the degree of liver fibrosis in CCL₄ liver fibrosis rats.The mechanism may be related to increase the expression of MMP-1 protein in liver tissue of CCL₄ rats with hepatic fibrosis,and down regulate the expression of TIMP-1 protein.