Study On The Chemical Constituents And Biological Activities Of Illigera Aromatica S.Z.Huang Et S.L.Mo

Illigera is a genus in the family Hernandiaceae,which contains approximately fifty-nine species from tropical Africa to Madagascar and East Asia.Illigera aromatica S.Z.Huang et S.L.Mo is a small liana mainly growing in Guangxi province,China.It is often used as a folk medicine,and its stems are often soaked in water by local people to treat tuberculosis and promote blood circulation.However,few phytochemical studies on the genus Illigera have been reported and only three species have been examined.Chemical investigation of the MeOH extracts from the stems of the Chinese plant Illigera aromatica has resulted in the isolation of four new natural aporphine alkaloids:laurodionine B?7?,N-formyllaurolitsine?8?,illigerine A?9?and illigerine B?15?as well as sixteen known products,palmitic acid?1?,?-amyrin?2?,succinic acid?3?,daucosterol?4?,sitosterol palmitate?5?,allantoin?6?,dehydroeburiconic acid?10?,glycyrol?11?,liquiritigenin?12?,2?,3?,24-dihydroxy-urs-12-en-28-oic acid?13?,isoliquiritigenin?14?,?-sitosterol?16?,liquiritin?17?,machigline?18?,norboldine?19?and actinodaphine?20?.The chemical structures of all compounds were determined by spectrometric analysis.Compounds 7,9,10,15,18 and 20 were tested their in vitro inhibitory activity against Bcap37 human breast cancer cells,SMMC7721 human liver cancer cells and Hela human cervical cancer cells.Laurodionine B?7?,illigerine A?9?,illigerine B?15?and machigline?18?displayed high cytotoxic activity against Hela human cervical cancer cells(IC₅₀ 11.77 ± 1.02 ?g/mL,18.39 ± 2.06 ?g/mL,12.40 ± 0.78 ?g/mL,19.56± 1.09 ?g/mL)respectively.Illigerine A?9?,laurodionine B?7?and machigline?18?were found to show moderate cytotoxicities against SMMC7721 human liver cancer cells(IC₅₀ 15.62 ± 1.75 ?g/mL,20.83 ± 1.80?g/mL,29.36 ± 1.53 ?g/mL)respectively.Illigerine B?15?exhibited moderate cytotoxic effects on Bcap37 human breast cancer cells(IC₅₀ 28.69 ± 1.80 ?g/mL)compared with other compounds(IC₅₀>30 ?g/mL).Dehydroeburiconic acid?10?and actinodaphine?20?exhibited weak cytotoxic activities against three tumour cells(IC₅₀>30 ?g/mL).In DPPH assay,actinodaphine?20?demonstrated good antioxidant activity(IC₅₀ value of 17.4 ± 0.8 ?g/mL),compared with the aerial parts W1,W2,W3 and W4(IC₅₀ value of 18.5 ± 1.2 ?g/mL,21.0±1.8 ?g/mL,23.6 ± 1.4 ?g/mL,52.9 ± 2.1 ?g/mL)respectively.This issue also summarized the chemical constituents and biological activities of the genus Illigera,and will support the scientific basis to the development and utilization of them.