Effects Of Apolipoprotein M On Expression Of Myocardium Inflammation Related Factors In Mice

Objective:To investigate the effects of apolipoprotein M（ApoM）on the expression of NF-κB,interleukin-1β（IL-1β）and intercellular adhesion molecule-1（ICAM-1）and interleukin-6（IL-6）mRNA levels in myocardium of mice.Methods:Using base quenched probe techniques,we confirmed the genetypes of mice as ApoM wild type(ApoM^+/+)or ApoM knockout type(ApoM^-/-).ApoM^-/-mice were randomly divided into control group and other 2 groups（1 h,3 hrs.）with 6 mice in each group.ApoM^+/+mice were also grouped in the same way.The mice of control group were not accepted any treatment.The mice in other 2 groups were randomly assigned to receive intraperitoneal injection of lipopolysaccharides（LPS）（2mg/ml）and mice were sacrificed at 1 h,3 hrs respectively.The myocardium tissues were collected and detected mRNA expression of NF-κB,IL-1β,IL-6 and ICAM-1 by Polymerase Chain Reaction.Eighteen ApoM^-/-male mice aged 6 to 8-weeks-old（approximately 23²5g body weight）and eighteen age and gender matched ApoM^+/+mice were applied in the present study.Results:With the effect of LPS,the levels of NF-κB,IL-1β,IL-6 and ICAM-1 in ApoM^-/-mice myocardium were significantly higher than ApoM^+/+mice myocardium,1h（all U values are 0.00;all P values are 0.00）;3h（U value:3.00,0.00,0.00,1.00;P value:0.015,0.002,0.002,0.004）.TWO-Way ANOVA showed that there was interaction between LPS and ApoM on NF-κB,IL-1β,IL-6 and ICAM-1 expression levels in mice myocardium.1h（F value:23.5,33.74,40.90,167.00;P value:<0.001,<0.0001,<0.0001,<0.0001）;3h（F value:6.092,27.91,12.66 and 12.06;P value:0.023,<0.0001,0.002,0.002）.Conclusions:ApoM inhibited LPS-induced NF-κB,IL-1β,IL-6 and ICAM-1expression in mice myocardium.