Experimental Observation For The Application Of Ethanol In Rabbit Sciatic Nerve And Vascular

Objective: The white rabbits were used as the subjects in this research.This research aim to explore the optium time for devitalization using ethanol in soft tissue sarcoma surgery.Under sterile conditions,the femoral vessels and sciatic nerve were exposed with outer layer surrounded by anhydrous alcohol for different time,the specimens were obtained and fixed immediately after treatment different time by ethanol.The function of the rabbits' lower extremities were observed and the specimens were investigated with microscopy to illustrate the injuries and repair of tissues after the devitalization for two,six and eight weeks,respectively.Methods: A total of 123 white rabbit were randomly divided to three groups,One group containing sixty subjects were used for sciatic nerve devitalization,and the second groups containing sixty subjects were used for femoral vessels femoral vessels devitalization,and the third group used as control.Under sterile conditions,he femoral vessels and sciatic nerve were exposed with outer layer surrounded by anhydrous alcohol for 5 min,10 min,15 min,20 min and 25 min,respectively,after the.complement of the treatment,the specimen were isolated and fixed immediately,and then sampled and fixed as previously after 2 weeks,6 weeks and 8 weeks,respectively.Comparative analysis of the function of the lower extremities and microscopy observation for the injury and repair status of the nervous and vessel tissues.Result: The impact of variant ethanol devitalization times is different.Histological observation of vessel and neural tissues suggested that the edema became more and more serious with the increasing the time of ethanol devitalization from 5 minutes to 25 minutes.The objects' vessel and neural tissues treated with ethanol from 5 minutes to 20 minutes could returned to normal8 weeks after surgery.However,the objects' vessel and neural tissues treated with ethanol for 25 minutes could not returned to normal,and the Symptom of thrombosis and ischemia were prone to present to the objects' vessel and neural tissues treated with ethanol for 25 minutes.Conclusion:To maximize the tumor devitalization rate of ethanol during the soft tissue sarcoma excision and ensure the repair of the vessel and nerve after surgery,20 minutes is the appropriate time for the devitalization with ethanol during theoperation of tumor excision.