The Expression And Significance Of AR And NCOA 1 In Bladder Cancer Tissues

Objective:1 To explore the role and significance of AR and NCOA1 in the initiation and development of bladder cancer,we studied the expression of androgen receptor(AR),nuclear receptor coactivator 1(NCOA1)in bladder cancer tissues,and the relationship between the expression of AR and NCOA1 with the clinicopathological features of bladder cancer patients.2 According to the expression of AR and NCOA1 in bladder cancer tissues,we investigated the relationship between AR and NCOA1 expression in bladder cancer tissues.Method:69 cases of bladder cancer patients who treated by radical resection were collectedfrom January 2014 to October 2017 in department of urology ofGanzhou Municipal Hospital and First Affiliated Hospital of Gannan Medical College.Bladder cancer tissues and adjacent tissues from bladder cancer patients were selected as research materials.The total protein of bladder cancer tissue and adjacenttissue was extracted,and the relative expression level of AR and NCOA1 in bladder cancer tissues and adjacent tissues weremeasured by Western blot.The bladder cancer tissues and adjacent tissues were sliced,the protein expression of AR and NCOA1 in bladder cancer tissues and adjacent tissues were detected by immunohistochemistry.According to the relative expression of AR and NCOA1 protein in patients with bladder cancer,the relationship between them and clinical characteristics were analyzed.Finally,the relative expression of AR and NCOA1 protein in bladder cancer tissues was analyzed by correlation analysis.Result:1.The Western blot result ofthe protein relative expression levels of AR and NCOA1 in bladder cancer tissues and adjacent tissues showed thatthe relative expression level of AR and NCOA1 protein in bladder cancer adjacent tissueswere 0.79(0.41,1.21),0.66(0.45,0.84),respectively,the relative expression level of AR and NCOA1 protein in bladder cancer tissueswere 1.92(1.23,3.06),1.60(0.89,2.50),respectively,Compared with the relative expression level of AR and NCOA1 protein in the adjacent tissue,the relative expression level of AR and NCOA1 protein in bladder cancer tissues was significantly higher than the relative expression level of AR and NCOA1 protein in the adjacent tissues.2.According to theimmunohistochemistry result ofthe protein relative expression levels of AR and NCOA1 in bladder cancer tissues and adjacent tissues,we found thatthe expression of AR and NCOA1 are mainly located in the nucleus,the high expression rates of AR and NCOA1 in bladder cancer tissues were 82.6%(57/69),73.9%(51/69),respectively;The expression rates of AR and NCOA1 were 26.1%(18/69)and 14.5%(10/69)in adjacent tissues,respectively;Compared with the high expression rate of AR and NCOA1 in the adjacent tissues of bladder cancer.The high expression of AR and NCOA1 in bladder cancer tissues were significantly higher than the high expression rate of AR and NCOA1 in theadjacent tissues of the bladder cancer.3.The relationship between the relative expression of AR and NCOA1 and the clinical characteristics of bladder cancerpatients was analyzed and found that the expression of AR protein was significantly correlated with pathological grading,clinical stage and lymph node metastasis in bladder cancerpatients,the expression of NCOA1 protein is significantly correlated with clinical stage and lymph node metastasis in bladder cancerpatients.4.According to the relative expression of AR and NCOA1 protein in bladder cancer patients,we found thatthe relative expression level of AR protein was positively correlated with the relative expression level of NCOA1 protein(r=0.661,p<0.001).Conclusion:1.The relative expression levels of AR and NCOA1 protein in bladder cancer tissues were significantly higher than those in adjacent tissues.2.The expression level of AR proteinin bladder cancer tissues is significantly correlated with pathological grading,clinical stage and lymph node metastasis of bladder cancerpatients.The expression of NCOA1 protein in bladder cancer tissuesis significantly correlated with clinical stage and lymph node metastasis of bladder cancerpatients.3.There are a correlation between the expression level of AR and NCOA1 protein in bladder cancer tissues.