Expression And Significance Of Angiogenesis Mimicry Proteins In Meningiomas

Meningiomas are common intracranial vascular benign tumor,which are high incidence in central never system tumors,only lesser than never glioma,accounts for about 15% ~ 20% of intracranial tumors.At present,though meningioma belongs to benign tumors,but part of the meningioma can still be seen around to normal brain tissue,and reappear easily after surgery,patients with poor prognosis.Adequate nutrition is a prerequisite for tumor growth and invasion,and meningioma is rich in solid tumor blood vessels,that the imbalances between factors of promoting angiogenesis and inhibiting angiogenesis is the precondition of meningioma formation.So the study of the molecular mechanism of meningioma angiogenesis will help to understand meningioma invasive biological behavior and the formation mechanism.Ischemia hypoxia is a key stage,the malignant tumor in the process of the development of the rapid growth need to increase the oxygen in its growth microenvironment,thus the formation of the pipeline structure is the premise.Vasculogenic mimicry(VM)is the pipeline structure while there is no endothelial cells,but cancer cells can enter pipeline line sample structure and simulate the angiogenesis,blood can flow in pipe structure in the endothelial cells.Studies had shown that calcium signaling pathway proteins related VM vascular epithelial adhesion(VE-cadherin)-epithelial cell kinase A2(Eph A2)-matrix metalloproteinases(MMPs)obviously existed in the VM reticular structure formation.But the forming process of VE-cadherin,VM Eph A2,the expression of MMP-9 in meningiomas and its clinical classification and the relationship between the literature had not been reported.This research of 56 patients with meningioma,analysis of VE-cadherin,Eph A2,the expression of MMP-9 in which the possible meaning and with the VM for nearly three years.To provide meningioma antiangiogenesis therapy,reduce the recurrence rate and postoperative auxiliary theoretical basis for molecular target therapy.Objective To explore the different levels of the patients with meningioma VM formation three important proteins(VE-cadherin,Eph A2,MMP-9),and microvascular density(microvessel density,MVD),,to discusses the contact of three proteins with meningioma VM formation,and to provide the theory basis for targeting antiangiogenesis therapy after surgery.Methods According to the nervous system tumor classification standards of world health organization(WHO)in 2014,56 patients with meningioma combined with imaging examination,intraoperative observation and postoperative pathologic classification.In 18 patients with craniocerebral trauma,the implementation of the standard big bone flap decompression resection of brain tissue for comparison,the structure changes of brain tissue were observed with HE staining;the proteins expression of VE – cadherin,Eph A2 and MMP-9 were detected in meningioma by immunohistochemical staining SP method and Western blot mothod;Microvascular density(MVD)detected by CD34 immunohistochemical SP method of 56 cases with meningioma in primitive hematopoietic cell antigen,and statistical methods to analyze VE-cadherin,the correlation between Eph A2 and MVD.Results Compared with normal group,MVD value increased obviously with the increase of level of meningioma,meningioma MVD value differences were significant(p < 0.05);Compared to grade I meningioma,VE-cadherin,Eph A2,MMP-9expression in class II and class III meningioma and MVD is related,and significant difference(r1 = 0.865,r2=0.728,r3 = 0.807,p < 0.01);In protein level,the expression levels of VE-cadherin,Eph A2 were higher in level ? meningioma than those in class ? and class ?,there were significant difference(p < 0.05).Conclusion: VE-cadherin,Eph A2,MMP-9 proteins promote meningioma formation and growth of new blood vessels,has certain influence on the growth of meningioma invasive,in the occurrence and progress of meningioma,VE – cadherin,Eph A2 and MMP-9 proteins involved in the VM formation,involved.MVD can reflect the invasive meningioma indirectly;Molecular targets of VE-cadherin,Eph A2,MMP-9 provides a theoretical basis of meningioma antiangiogenesis therapy.