Mechanism Of Tianjingbusui And Huoxue Huatan Kaiqiao Decoction Begin To Understand Law Of VD Rat Hippocampus Of 5-HT Levels Effect

Objective:To use modern Elisa technique to explore the method of filling fillingpulp,invigorate the circulation of phlegm and begin to understand the effectiveness of thevascular dementia rats and the mechanism of action.Method: 1.Choose the SPF Wistar rats,half male and female,weight 220 + 20 g,a total of 60,randomly divided into: control group,model group,positive medicine contrast many fill out fill pith,invigorate the circulation of phlegm begin to understand group,phlegm begin to understand new green particles in high dose group,middle dose group,low dose group,a total of six groups,each group of ten only.Adopt double vascular obstruction(2VO)improvement method to copy the vascular dementia rats model.2.The platform test: using platform experiments to detect vascular dementia ratsbehavior change.Inspection method of filling filling pulp,invigorate the circulation of phlegm and begin to understand the influence of the vascular dementia rats learning and memory ability.3.Using elisa method to detect type dementia rat hippocampus 5-HT levels serotonin.Result: 1.Platform experiment results show that filling filling pulp,invigorate the circulation method of eliminating phlegm to begin to understand the high dosage group and positive control drug aricept group can reduce the number of VD model rats platform experimental error(P < 0.05),to extend the platform test incubation period(P < 0.05).Positive control group and high dose of Chinese traditional medicine group there was no significant difference(P > 0.05).2.High dose of Chinese traditional medicine group play the imagitional of the hippocampus of the rat vascular dementia 5 high serotonin levels compared with model group,statistically significant(P<0.05).Positive drug Donnelly,pp qi group VDrathippocampus 5 serotonin levsls higher than the model group.statistically significant(P<0.05).Comppared with positive drug grou,high dose of Chinese traditional medicine grouo no statistcal difference(P>0.05).Conclusion: Mechanism of tianjingbusui and huoxuehuatan kaiqiao begin to understand law fornula can obviously inmprove the vascular dementia rats learning and memory ability,can increase the hippocampus 5 serotonin levels,thus reducing the vascular dementia rats cell death.