Study On The Prevention And Treatment Of Desmodium Renifolium In Chronic Renal Failure Rats

Objective:1.An important method will be recognized by the mass through looking for new drugs,and richful medicinal planting resources deposit in ethnic minority medicine,Desmodium renifolium is one of Dai Medicine.A modern pharmacologic method is used to develop the drug,and in order to establish a basis for finding and developing new drugs.2.Kidney-tonifying is significant in clinical effect kidney,but so far,Dai doctors of XISHUANGBANNA use the drug,according to clinical experiences,and its pharmacodynamics research has not been reported.The experiment of Desmodium renifolium studies the effect of nourishing kidney,and which can state the pharmacodynamics effect of the medicine,and study the system of chronic renal failure,and provide an experimental method for the medicine developing and using.Methods:1.Preparing medicinal materials.In this study,water extraction and alcohol extraction were used to extract and prepare the sample.2.The CRF model selection and copy.Adenine was used to establish animal model of chronic renal failure?CRF?,and to screen the optimal concentration and administration period.3.Experienced formula effect from the folk in CRF ratsIn the experiment,preventive administration was used to fill the stomach,and kidney-tonifying prescription was screened by detecting the changes of indexes in rats.4.The effect of Desmodium renifolium in CRF rats modelUsing HE staining observes pathological changes in the rat model of chronic renal failure kidney.5.Observing CRF rats model induced by blood RBC,HGB,Plt,Mcv,Mpv.Using blood analyzer is to test rats'RBC,HGB,Plt,Mcv,Mpv.6.The effect of Desmodium renifolium in CRF rats model induced by Scr and BUN in serum.Using Scr checked serum of rats.Using automatic biochemical analyzer determined the serum BUN.7.The effect of Desmodium renifolium in CRF rats model induced by 24 hours urine and the influence of the SG,Ucr,Ccr,PRO,?1-MG and KIM-1.Using Ucr tested Ucr of rat urine;Urine analyzer is used to test the urine of rats SG,PRO;Using the following formula^[12]calculate Ccr:Ccr=Ucr?24h urine volume/Scr?1440Enzyme linked immunosorbent?ELISA?in rat urine?1-MG and KIM-1.8.The effect of Desmodium renifolium in CRF rats model induced by the local tissue AQP2?TGF-?1?HIF-1?and CTGF expression.Immunochemical tissue staining method is used to observe the rat kidney tissue AQP2?TGF-?1?HIF-1?and CTGF positive expression,and analysis is used by the Image-proplus 6.0 image.Result:1.Preparation of the test sample.The experimental drugs were Dai prescription1,Dai prescription2,and Dai prescription3and Desmodium renifolium,and Dai prescription 3;Dai prescription1 is divided into water extraction and alcohol extraction;Dai prescription2 and Dai prescription3 are water extraction;Desmodium renifolium was divided into water extraction and alcohol extraction,due to different extract method,and alcohol extraction group is divided into high,medium and low three dose groups.Seven groups:Each extract concentration and dosage:Dai medicine prescription1 is divided into water extraction and alcohol extraction,the extraction of mixed suspension drug concentration is 0.41g/mL,drug doses were 4.14g/kg;Dai medicine prescription2 of suspension liquid extraction and concentration were 0.28g/mL,crude drug is2.79g/kg;Crude drug concentration of the extraction of Dai prescription 3 were 1.70g/mL,crude drug were 17.01g/kg;Desmodium renifolium was divided into water extraction and alcohol extraction,the extraction of mixed suspension is 0.14g/mL,crude drug were 1.35g/kg.After screening of Desmodium renifolium,the high is?5.4 g/kg?,the middle is?2.79g/kg?and the low is?1.35g/kg?.2.CRF rats model selection and copy.The concentration of rat model is 1.0%,during 15days setting model,renal color of normal group is smooth,no swelling,and membrane is easy to strip;renal size ofmodel group increased,color and the surface is granular,severe cases are"great white kidney",coated is not easy to shed.Under a microscope,glomerular of the normal group rat and form of renal tubular are good,the normal cortex and medulla boundary is clearly visible;the number of model group rats glomerulus atrophy decreased,renal tubular is expansion,brown sediments is visible.3.The effect of National medicine experienced formula in rat kidney pathological model.?1?Alcohol extraction Dai medicine prescription1 and Desmodium renifolium have the same effect on chronic renal failure rats model.But water extraction of Dai medicine prescription1,Dai medicine prescription2 and Desmodium renifolium in edema of CRF rats model improve unobviously.Dai prescription 3 of chronic renal failure induced by weak inhibitory effect.?2?The results of SCR and BUN in serum showed that model group were significantly increased?p<0.001?.BUN and SCR decreased in different degrees in each drug group,and BUN and SCR decreased most significantly in positive drug group and Dai drug group?p<0.001?.4.Pathological changes of Desmodium renifolium kidney rat kidneyDesmodium renifolium dose group can improve pathological tissue in the rat model of chronic renal failure,and the high dose group compared with the model group,and significantly reduce renal tubular expansion,and the result of positive medicine group is similar.5.The influence of Desmodium renifolium in Serum BUN,and Scr and Ccr induced by chronic renal failure?1?Blood tests show:RBC,HGB of Model group are significantly lower than the normal control group?P<0.01?;RBC:Positive medicine group and the treatment group are higher than model group,the differences between the two groups have statistical significance?P<0.05?,.?2?Serum BUN shows:BUN level in model group was obviously higher than normal control group?P<0.001?;Positive drug group,high and low dose group,medication BUN level is lower than the model group.Statistical significances between the two groups were significant?P<0.01?.The BUN level of middle dose group was also significantly reduced?P<0.05?.?3?Serum Scr shows:the Model group is significantly higher than serum Scr?P<0.001?;Scr levels of each dose group have decreased,Positive medicine,highandmiddledosegrouphavean extremelysignificantdifference?P<0.001?,Low dose group has significant difference?P<0.01?.?4?Serum Ccr shows:Ccr of model group was significantly reduced;Ccr of High dose group was significantly increased?P<0.01?;in the positive medicine,middle and low dose groups compared with the model group;there was no significant difference in Ccr level.6.CRF of Desmodium renifolium in rats model induced by urine.Routine urine shows:Compared with normal control group,24h urine of model group was significantly increased?P<0.001?,SG lower?P<0.001?,Urine PRO is positive.Positive medicine group and SG of Each dosage group increased,Urine PRO is negative.7.Effects of.Desmodium renifolium on renal injury markers in rats with experimental chronic renal failureThe result of kidney injury markers in each group shows that the levels of?1-MG and KIM-1 in the model group were significantly higher than those in the control group?P<0.001?,the positive drug group and each drug group were significantly reduced?P<0.001?.8.The effect of Desmodium renifolium group on CRF rats model induced by the local tissue AQP2?TGF-?1?HIF-1?and CTGF.Desmodium renifolium group can obviously improve the pathological organization form of chronic renal failure rats.AQP2 expressed in model group significantly?P<0.05?,Positive medicine group and Desmodium renifolium group is improved significantly;TGF-?1?HIF-1?and CTGF of Model group expression are rised,the positive medicine group and treatment group reduced,and TGF-?1,the high group compared with model group have significant meaning?P<0.01?.Conclusions:Through replicating chronic renal failure model,screening Dai folk experienced formula in chronic renal failure,the experimental results show that:Compared with other prescriptions,Desmodium renifolium has the best effect in preventing and treating experimental chronic renal failure in rats.Subsequently,Desmodium renifolium was further analyzed,the comprehensive results showed that the high-dose group had obvious effect on the prevention and treatment of chronic renal failure.The mechanism is related to improving renal anemia,reducing serum BUN?Scr level,increasing SG,decreasing urinary?1-MG and Kim-1 level,increasingAQP2expression and decreasingTGF-?1,HIF-1?and CTGF expression in CRF rats.