| Objective:To study the relationship between the autophagy of human leukemia K562 cells induced by diallyl disulfide?DADS?and the PI3K/Akt signaling pathway.Methods:K562 cells were treated with 20,40 mg.L-1 DADS for 24h or K562cells were treated with 40 mg.L-1 DADS for 12,24h.After treatment,the expression levels of Akt,p-Akt,P70S6K,p-P70S6K and LC3 protein were detected by western blotting.Treated K562 cells with 40mg.L-1 DADS,Akt inhibitor?MK-2206?alone or two drug combined treated K562 cells for 24h,the expression levels of Akt,p-Akt and LC3 protein were detected by western blotting.Control groups were used as references.Results:1.The expression of p-Akt and p-P70S6K protein in K562 cells treated with 20,40 mg.L-1DADS were significantly lower than that of the control group?P<0.05?.The total protein Akt and P70S6K were no significant difference?P>0.05?.The expression level of autophagy-related protein?LC3?was higher than that of the control group?P<0.05?,and the highest in the 40mg.L-1DADS group?P<0.05?.2.The relative expression of p-Akt and p-P70S6K protein in K562 cells treated with 40mg.L-1DADS for 12,24h was significantly lower than that in control group?P<0.05?.The expression of LC3 protein was significantly higher than that of the control group?P<0.05?,and the expression of Akt,P70S6K were not significantly different?P>0.05?.3.In the DADS group,pathway inhibitor group and the combination group the relative expression levels of p-Akt,p-P70S6K protein were both lower than the control group?P<0.05?;The expression of total Akt,P70S6K protein were not significantly different?P>0.05?.In the DADS group,pathway inhibitor group and the combination group the relative expression levels of LC3protein was significantly higher than that of the control group?P<0.05?and the combination group was the highest group?P<0.05?.Conclusion:DADS induces the autophagy of K562 cells,and its mechanism is related to DADS inhibits the activation of key proteins Akt,P70S6K in the PI3K/Akt signaling pathway. |
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