The Role Of The MEK/ERK Signaling Pathway In Dads-induced Autophagy Of K562 Cells

Objective:To investigate the effect of MEK-ERK signaling pathway on diallyl disulfide-induced autophagy in human leukemia K562 cells.Methods: K562 cells were divided into experimental group,blank control group and Vehicle control group.K562 cells in the experimental group were cultured with 10,20,40 mg/LDADS for 48 hours.The morphological changes were observed by inverted microscope.monodansylcadaverine(MDC)staining was used for detecting the formation of the autophagic vacuoles(AV),and the rates of autophagy were analyzed by flow cytometry.The protein level of MEK,phosphoMEK(p-MEK),ERK,phospho-ERK(p-ERK),LC3?/? were detected with western blot.Results:With the increasing of DADS concentration,the number of K562 cells was decreased significantly,and the morphology of some K562 cells became irregular and membrane deformed.The staining of cells and the number of green spots in the cells increased,suggesting that autophagy of the K562 cells cultured with DADS increased.The autophagy rates of K562 cells increased gradually after cultured with DADS for 48 h,the autophagy rate in 40 mg/L DADS group was the highest,and the autophagy rates in 20,40mg/L DADS group were higher than that in the blank control group(P < 0.05).There were no significant difference in the expression level of MEK and ERK protein between each group(P > 0.05),with the increasing of DADS concentration,the protein expression of p-MEK,p-ERK and LC3?/LC3? increased,The difference between the experimental group and the control group was statistically significant(P < 0.05).Conclusion:DADS may induce autophagy of K562 cells by activating MEK-ERK signaling pathway.