Biocompatibility Analysis Of Carboxymethyl Chitosan/Silk Fibroin Blend Porous Scaffold

As two of the most widely used biomaterials,silk fibroin(SF)and carboxymethyl chitosan(CMCS)have been synthesized and used in many studies to produce biofilms or scaffolds,Mainly because both have better cell compatibility,biodegradability and non-toxic characteristics.In the previous study,carboxymethyl chitosan and silk fibroin were mixed into porous biological scaffolds,combined with the different mechanical and biological properties of the two,to prepare a bionic extracellular matrix of biological stent,more conducive to cell growth,(CMCS)were prepared by mixing fresh silk fibroin solution / silk protein nanofibers(SF / SN)= 15: 1,and the porous bio-scaffolds were prepared by freeze-drying method.The scaffolds were then cross-linked with EDC-NHS-methanol solution to prepare a scaffold with nanofiber microstructures and protein-polysaccharide complexes similar to the extracellular matrix and insoluble in water.The results showed that SF / CMCS mixed stent had good porosity and thermostability,and the results showed that the SF / CMCS mixed stent had good porosity and thermostability.The results showed that SF / CMCS mixed stent had good porosity,Sex,swelling rate,and in the CMCS / SF = 1: 4 when the stent is most conducive to cell proliferation.On the basis of the above experiment,different proportions of stents were implanted into SD rats subcutaneously,and removed at 1 week,2 weeks,4 weeks and 8 weeks,sliced,stained,immunohistochemistry,mason and HE staining,Vascular growth and inflammatory cell infiltration,the use of SPASS statistical software,the average of the data set using a one-way variance.The results showed that the number of inflammatory cells and blood vessels increased gradually with time,which was statistically significant.In summary,SF / CMCS mixed stent has good biological compatibility,with a certain repair soft tissue potential.