The Application Of NGAL In Exosomes Derived From Urinary In The Diagnosis Of Renal Carcinoma

Objective: This study intends to compare the expression of NGAL in urine exosomes between normal and renal cancer patients to determine the value of NGAL in urinary excretion in the diagnosis of renal cell carcinoma and lay a foundation for the search for a good biomarker for the diagnosis of renal cell carcinoma.Methods: Fresh morning urine specimens were collected from patients with renal cell carcinoma and normal healthy individuals.Urinary sediments and exosomes were isolated.The exosomes were identified by surface marker by western blot,transmission electron microscopy,and nanoparticle size analysis.Westblot detected NGAL levels in urine sediments and exosomes in patients with renal cell carcinoma and normal controls.NGAL mRNA lever of urinary sediment and exosomal in renal cell carcinoma and normal controls were detected by Real-time fluorescence quantitative PCR analysisResults:(1)We Successfully extracted urine exosomes from patients with renal cell carcinoma and normal healthy persons,and successfully identified exosomes in urine via TEM,Wsetblot and nanoparticle size analysis;(2)NGAL levels of urinary exosome in patients with renal cell carcinoma were significantly higher than those in normal human urine exosomes(p<0.05).(3)NGAL mRNA levels of urinary exosome in patients with renal cell carcinoma were significantly higher than those in normal human urine exosomes.(p<0.05).Conclusion: NGAL in urine exosomes derived from healthy humans and patients with renal cell carcinoma is differentially expressed.Urinary excretion of NGAL may be used as a biomarker for diagnosis of renal cancer.This study shows the possibility of using urine exosomes as biomarkers of human urinary tract diseases and may contribute to its role in understanding the pathophysiology of renal cancer.