Preliminary Study On The Effect Of Chromium On Proliferation Of Prostate Cancer Cells

ObjectiveBy screening for the promotion of prostate cancer PC3 cell line value-added effect of chromium valence,concentration and exposure time.To further explore the effect of low dose chromium on the biological behavior of its cell lines,and to explore its mechanism.MethodsThe effects of different concentrations of chromium on PC3 cell lines were detected by MTT assay and CCK-8.The dosage and exposure time of chromium reagent and chromium were determined according to the experimental results.The proliferation effect of low dose chromium exposure on prostate cancer PC3 cells was further confirmed by monoclonal assay according to the related valence,concentration and duration of time of MTT and CCK-8.The effects of low dose of chromium on the biological behavior of PC3 cell line in prostate cancer were determined by cell migration,cell invasion,apoptosis and cell cycle.Mouse tumor formation experiments to further study the effects of chromium exposure on prostate cancer in vivo;The mechanism of low dose of chromium exposure to promote the proliferation of prostate cancer cells was further explored by human genome sequencing,and the mechanism was explored by Western Blot.ResultsMTT,CCK-8 experiments showed that 0.4umol / L potassium chromate exposure to 48 hours after the prostate cancer cells significantly promote the proliferation effect.Monoclonal formation experiments confirm this result.0.4umol /L potassium chromate exposure 48 hours after the promotion of prostate cancer cell migration,cell invasion,cell apoptosis and cell cycle no significant effect.By mouse tumorigenesis experiments,5 mg / kg / day of potassium chromate promoted tumor growth in mice.The prostate cancer cells were treated with untreated prostate cancer cells for 48 hours after exposure to 0.4 μmol / L potassium chromate.The results showed that there were differentially expressed genes in the specimens treated with0.4umol / L potassium chromate,and the differences were expressed in biologicalprocesses,cell components and molecular mechanisms.A total of 759 different genes(387 up-regulated genes,372 Down-regulated genes).Western Blot showed that the expression of Vimentin in PC3 cells was promoted by 0.4umol / L potassium chromate treatment,suggesting that low dose of chromium exposure promoted the EMT of PC3 cells in prostate cancer,which confirmed that low dose of chromium exposure could promote the proliferation,migration and invasion of prostate cancer cells.ConclusionLow dose chromium exposure promotes proliferation,migration and invasion of prostate cancer cells and promotes tumor growth in vivo.There are different genes in biological process,cell composition and molecular mechanism.Vmentin can promote the expression of PC3 in prostate cancer cell line EMT,and then affect the proliferation,migration and invasion of prostate cancer cells.