MiR-154Controls Cells Proliferation By Targeting CCND2in Prostate Cancer Cell Lines

Background:MicroRNAs (miRNAs) are a class of about20to22nucleotides short noncoding single-stranded RNA that are important regulators of gene expression at the posttranscriptional level by degrading or repressing target miRNAs.A lot of evidence prove that microRNAs play a important role in development, differentiation, apoptosis and proliferation of the prostate cancer.In prostate cancer, the study about the miR-154expression and function is little,.In this study,we demonstration the expression and function of miR-154in prostate cancer cells.Methods:The qRT-polymerase chain reaction (PCR) detection expression levels of miR-154in27cases of prostate cancer and9cases of normal prostate tissue samples.Using cell proliferation experiment, the clone formation experiment, cell cycle and Western blot analysis to evaluate the function of miR-154and CCND2changes impact on prostate cancer cell line PC3and DU145. Dual-luciferase reporter assay was used to identify binding sites between miR-154and CCND23’-UTR.Results:Compared with the normal prostate tissue,miR-154expression level of prostate cancer tissue significantly lowered.In vitro,we use experiments CCK-8, colony forming experiment, cell cycle analysis to demonstration the function of miR-154and CCND2.,miR-154up-regulation or knock down CCND2can significantly inhibit the proliferation of prostate cancer cells. Dual luciferase experimental results show CCND2is the direct target genes of miR-154. Conclusions:MiR-154in prostate cancer can be used as a tumor suppressor, regulate the expression of CCND2directly after the transcription level.Increase the expression of miR-154or knock down CCND2level can affect prostate cancer cell proliferation ability.MiR-154is expected to become a molecular marker for prostate cancer diagnosis and treatment.