| Objective: To explore the expression of micro RNA-506 in serous ovarian cancer and the mechanism of epithelial mesenchymal transition in ovarian cancer.Methods: 1.Collect 43 patients with serous ovarian cancer(SOC)for standard surgical treatment between December 01,2006 and December 01,2015 in our hospital as research group,while cottecting10 cases of benign serous ovarian tumor as control group.By the quantitative Real-Time Polymerase Chain Reaction(q RT-PCR),the expression of miRNA-506 was detectded in serous ovarian cancer and benign serous ovarian tumor tissues,the clinical pathological parameters were analyzed according to the miRNA-506 expression situation.2.Select A2780 cell line of the human ovarian cancer with high expression of miRNA-506 and SKOV3 cell line of the low expression of miRNA-506 as the research object,through the method of liposome transfection in A2780 cell lines were transiently transfected with miRNA-506 inhibitor and in SKOV3 cell line transfected miRNAS-506 mimics,then the expression of E-cadherin and Vimentin(the markers of epithelial mesenchymal transition)were detected in transformation cells by Western Blotting method to analysis the mechanism of miRNA-506 in epithelial mesenchymal transition of ovarian cancer.Result: 1.The comparison of miRNA-506 expression in serous ovarian cancer 1.1 The comparison of miRNA-506 expression in serous ovarian cancer and benign serous ovarian tumors The relative expression level of miRNA-506 in serous ovarian cancer was 19.74(mean rank),and the relative expression level of miRNA-506 in benign serous ovarian tumors was 33.22.The relative expression of miRNA-506 in serous ovarian cancer was lower than that in benign serous ovarian tumors,and the difference was statistically significant(Z=2.81,P=0.004 < 0.01). 1.2 The comparison of miRNA-506 expression in different stages of FIGO in serous ovarian cancer The relative expression level of miRNA-506 in patients with FIGO stage I and II of serous ovarian cancer was 28.86,and the relative expression level of miRNA-506 in patients with stage III and IV was 15.45.The relative expression of FIGO stage I and II serous ovarian cancer tissues was higher than that of stage III and IV,and the difference was statistically significant(Z=3.50,P=0.000 < 0.01).1.3 The comparison of miRNA-506 expression in different pathological grades of serous ovarian cancer The expression level of miRNA-506 in advanced serous ovarian cancer was 11.85,and the relative expression level of miRNA-506 in low grade serous ovarian cancer was 26.40.The relative expression level of miRNA-506 in advanced serous ovarian cancer tissues was lower than that in low grade serous ovarian cancer,the difference was statistically significant(Z=3.49,P=0.000 < 0.01).1.4 The comparison of miRNA-506 expression in different lymph node metastasis of serous ovarian cancer The expression level of miRNA-506 in patients with lymph node metastasis of serous ovarian cancer was 11.20,and the relative expression level of miRNA-506 in patients without lymph node metastasis was 25.27.The relative expression level of miRNA-506 in patients with lymph node metastasis was lower than that without lymph node metastasis,the difference was statistically significant(Z=3.11,P=0.001 < 0.01).1.5 The comparison of miRNA-506 expression in in serous ovarian cancer with different CA125 levels The expression level of miRNA-506 in patients with CA125>500U/ml group of serous ovarian cancer was 12.93,and the relative expression level of miRNA-506 in patients with CA125?500U/ml group was 26.38.The relative expression level of miRNA-506 in patients with CA125>500U/ml group was lower than that CA125?500U/ml group,the difference was statistically significant(Z=3.29,P=0.001 < 0.01).2.The comparison of epithelial mesenchymal transition markers expression in ovarian cancer cell lines 2.1 The expression of EMT markers Vimentin and E-cadherin in SKOV3 cell line transfected with miRNA-506 mimics 2.1.1 The comparison of Vimentin expression The vimentin expression of miRNA-506 mimics group was 0.0043±0.0087,blank group was 0.0670±0.0016,negative control group was 0.0699±0.0011,there was significant difference in the Vimentin expression of three samples(F=1.141,P= 0.00< 0 0.01).And the miRNA-506 mimics group and blank control group,and negative control group also were significantly different(P < 0.01),the difference between the control group and negative control group was not statistically significant(P > 0.05).The vimentin expression of miRNA-506 mimics group was lower than that of control group and negative control group.The result showed that the expression of Vimentin was decreased after the expression of miRNA-506 increased in ovarian cancer cell line.2.1.2 The comparison of E-cadherin expression The E-cadherin expression of miRNA-506 mimics group was 0.0024±0.0002,blank group was 0.0013±0.0003,negative control group was 0.0014±0.0001,there was significant difference in the E-cadherin expression of three samples(F=32.828,P= 0.001<0.01).And the miRNA-506 mimics group and blank control group,and negative control group also were significantly different(P < 0.01),the difference between the control group and negative control group was not statistically significant(P > 0.05).The E-cadherin expression of miRNA-506 mimics group was higher than that of control group and negative control group.The result showed that the expression of E-cadherin was increased after the expression of miRNA-506 increased in ovarian cancer cell line.2.2 The expression of EMT markers Vimentin and E-cadherin in A2780 cell line transfected with miRNA-506 inhibitor 2.2.1 The comparison of Vimentin expression The vimentin expression of miRNA-506 mimics group was 0.0232±0.0101,blank group was 0.0026±0.0009,negative control group was 0.0037±0.0011,there was significant difference in the Vimentin expression of three samples(F=424.965,P= 0.00<0.01).And the miRNA-506 mimics group and blank control group,and negative control group also were significantly different(P < 0.01),the difference between the control group and negative control group was not statistically significant(P > 0.05).The vimentin expression of miRNA-506 mimics group was higher than that of control group and negative control group.The result showed that the expression of Vimentin was increased after the expression of miRNA-506 decreased in ovarian cancer cell line.2.2.2 The comparison of E-cadherin expression The E-cadherin expression of miRNA-506 mimics group was 0.3278±0.0124,blank group was 0.0013±0.0003,negative control group was 0.0014±0.0001,there was significant difference in the E-cadherin expression of three samples(F=102.275,P= 0.00<0.01).And the miRNA-506 mimics group and blank control group,and negative control group also were significantly different(P < 0.01),and there was statistically significant difference between the control group and negative control group(P < 0.01).The E-cadherin expression of miRNA-506 mimics group was lower than that of control group and negative control group.The result showed that the expression of E-cadherin was decreased after the expression of miRNA-506 decreased in ovarian cancer cell line.Conclusion: 1.The expression of miRNA-506 in serous ovarian cancer tissue was significantly down compare with benign serous ovarian tumors,the relative expression of miRNA-506 in serous ovarian cancer was decreased with FIGO stage increased,pathological grade enhanced and lymph node metastasis occurred and CA125 level raised,suggesting that miRNA-506 may inhibit the occurrence,development and metastasis of serous ovarian cancer.2.The vimentin expression was decreased and E-cadherin expression was increased after the expression of miRNA-506 increased in serous ovarian cancer cell lines on the contrary,the expression of vimentin was increased and the E-cadherin expression was decreased after the expression of miRNA-506 decreased,suggesting that miRNA-506 is involved in the regulation of the EMT process,which may play an important role in the development and invasion of ovarian cancer.. |
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