The Effect Of Tumor Suppressor Gene PTEN On Mantle Cell Lymphoma Jeko-1

Mantle cell Lymphoma(MCL)is a very rare type of b-cell lymphoma,with its own unique clinicopathologic features.MCL relapse easily,is the lowest long-term survival rate of lymphoma subtype,median survival time for the 3-5.High degree of mantle cell lymphoma is a malignant,pathological histology and diverse,non-Hodgkin's b-cell lymphoma prognosis.The mechanism of molecular genetics of complex,although new genetic mechanisms continue to be discovered,but the nature of the lack of in-depth understanding Clinical manifestations of invasive,disease progress faster,more extensive lymph node involvement,extra-nodal disease common in liver,spleen and peripheral blood and bone marrow infiltration,usually associated with multiple myeloma involving the gastrointestinal tract and nervous system-dip.There is no standard first-line treatment of the disease,intensive chemotherapy and autologous hematopoietic stem cell transplantation improves efficacy,but still no cure.From pathogenesis factors related to figure out poor prognosis of MCL,and targeted to find targets,according to the target for new drugs,experimental and clinical validation select the best drug,is currently one of our refractory MCL research focus.PTEN gene was first discovered so far have phosphatase activity of tumor suppressor genes,the study found that tyrosine phosphorylation of PTEN may through the inhibition,inhibition of cell proliferation,while involved in cell cycle regulation,mutation,loss,abnormal protein expression,and may ultimately lead to cancer.PTEN has become especially Lymphoma hematopoietic system cancer research focus of Oncology,but in mantle cell lymphoma research,PTEN gene and pathway on mantle cell lymphoma and what biological characteristics and molecular mechanisms need to be further studied.Study finds that PTEN mutations can be detected in a variety of benign and malignant tumors and defects,lymphoma can also be detected in the abnormal expression of PTEN.This project through strengthening the exogenous PTEN gene expression,to inhibit the P13K-Akt-m TOR signaling pathway in another way,antitumor research on mantle cell lymphoma cells.Objective:To study the effect of tumor suppressor gene PTEN on mantle cell lymphoma Jeko-1.Methods :(1)Mantle cell lymphoma cell was transfected with pc DNA3.1-PTEN by lipofectamine.(2)Cell viability was detected by MTT assay.(3)Cell cycle stages and apoptosis were examined by flow cytometric analysis.(4)The phosphorylation levels of AKT and PTEN were evaluated by western blot analysis.Results:(1)pcDNA3.1-PTEN inhibited the proliferation of mantle cell lymphoma cell.(2)pc DNA3.1-PTEN contributed to the apotosis of mantle cell lymphoma cell.(3)pc DNA3.1-PTEN reduced the phosphorylation levels of AKT.Conclusion :The stably expression of PTEN inhibited the phosphorylation levels of AKT and restrained the PI3K/AKT/Mtor signal pathway,leading to the apotosis and the proliferation of mantle cell lymphoma cell.