Research Of The Correlation Between HPV Infection And Other Common Cervical Pathogen Infections In The Progression Of Cervical Lessions

ObjectiveTo investigate the infection status and correlation between the HPV and other common pathogens such as pathogenic bacteria,Ureaplasma urealyticum,Myccoplasma hominis,and Chlamydia trachomatis in different degree of cervical lession,and furthermore to explore its relationship with the progression of cervical lesions.To detect the expression of uPA in HPV infected cervical cancer tissue with different pathogen infection status in order to disscuss the role of HPV and pathogenic bacteria co-infection in cervical cancer.MethodsWe selected 424 patients with CIN or cervical cancer from January 2012 to January2016 in maternal and child health hospital as study subjects,of those 200 cases were divided into cervical cancer group,and 85 cases in CINI group which belongs to cervical low-level lesions(LSIL),56 cases in CINII group and 83 cases in CINIII group which belong to cervical high-level lesions(HSIL)or CINII group,CINIII group and cervical cancer group were divided into HSIL and above groups;Control group was matched for age,including 458 cases of patients with gynecological benign diseases treatment.All cases were taken cervical exfoliated cells for HPV genotyping detection using PCR-reverse dot hybridization technique,at the same time they were taken the cervical secretion for bacteria,fungi anaerobic bacteria conventional cultivation,UU,MH detection using liquid culture method,and CT detection using immune chromatography method.And using reverse transcription polymerase chain reaction(RT-PCR)technology to detect the uPA-mRNA expression of HPV infected cervical tissue,those including the group of 20 cases of cervical cancer with pathogenic bacterial infected,thegroup of 20 cases of cervical cancer without pathogenic bacterial infected and the group of 17 cases of normal cervix(normal control group)1 The relationship between cervical pathogenic bacterial,UU,MH,CT infections and cervical lesionsIn control group the infection rates of pathogenic bacterial,UU,MHandCT were15.9%,9.2%,5.5%and3.5%.In study group the infection rates of pathogenic bacterial,bacterial,UU,MH,andCT were 37.0%,28.1%,8.5% and18.4%.The infection rates.of pathogenic bacterial,UU,CT in study group were higher than that in the control group respectively,and the difference was statistically significant(P<0.01),while the infection rate of MH between the two groups had no statistical significance(P>0.05).The infection rates of pathogenic bacterial,UUand CT were 40.4%,29.8%,20.1% in HSIL and above group.The infection rates of pathogenic bacterial,UU and CT in HSIL and above group were higher than that in the control group,and the difference was statistically significant(P<0.01).The infection rates of pathogenic bacterial,UUand CT in LSIL group were 23.5%,21.2% and 11.8%.The infection rate of UU and CT in LSIL group were higher than that in the control group and the difference was statistically significant(P<0.05).Compared the infection rate of pathogenic bacteria between LSIL group and control troup,the difference had no statistical significance(P>0.05).The infection rate of pathogenic bacterial in HSIL and above group was higher than than that in LSIL group and the difference were statistically significant(P<0.05).Compare the infection rates of UU and CT between HSIL and above groups and LSIL group,the difference had no statistical significance(P>0.05).259 strains pathogenic bacteria had been detected in 882 cases of the research object.In cervical cancer group,89 strains pathogenic bacteria had been detected,which was given priority to with E.coli,accounted for 41.6%;In CIN group 88 strains pathogenic bacteria had been detected,which was given priority to with E.coli,accounting for 30.7%;In Control group 82 strains pathogenic bacteria had been detected,which is given priority to with E.coli too,accounting for 35.3%.Thepathogenic bacteria flora distribution difference in the cervical cancer group,CIN group and the control group has no statistical significance(P>0.05).2 Correlation analysis between other common pathogens infection and HPV infectionPathogenic bacteria infection was positively associated with HPV infection(r=0.232,P<0.01);UU infection was positively associated with HPV infection(r=0.267,P<0.01);CT infection was positively associated with HPV infection(r=0.213,P<0.01);But there was no correlation between HPV infection and MH infection(P>0.05)3 Relationship between the cervical other common pathogens and cervical lesions in different HPV infection casesLayered on HPV different situations,in HPV positive group,the infection rate of pathogenic bacteria,UU,CT were differences between the study group and the control group(P<0.05);In HPV negative groups,the infection rate of pathogenic bacteria,UU in the sudy group was no statistically significant difference with the control group(P>0.05),but the infection rate of CT in two groups difference was statistically significant(P<0.05).4 The level of uPA-mRNA expression in different groups with HPV infectionThe level of uPA-m RNA expression in cervical cancer group without pathogenic bacteria infection and cervical cancer group with pathogenic bacteria infection was higher than that in normal cervix group(P<0.05).The level of uPA-mRNA expression in cervical cancer group with pathogenic bacteria infection was higher than that in cervical cancer group without pathogenic bacteria infection(P<0.05).Conclusion1 Pathogenic bacteria infection in cervical was associated with CIN/cervical cancer,and was positively associated with increased degree of.cervical lesions.UUandCT infection was associated with CIN/cervical cancer,but has nothing to do with increased degree of.cervical lesions.MH infection has nothing to do with cervical lesions.2 Pathogens bacterial infections,UU infection and CT infection in cervical was coordinated infection of HPV in cervical lesions.Chlamydia trachomatis was the risk factors of cervical lesions.3 Pathogenic bacteria in patients with cervical cancer and CIN was given priority to with E.coli,but there was no difference in the flora distribution and normal cervix.4 Pathogenic bacteria infections may increase the expression of the uPA through collaborative HPV to cause cervical cancer.