Expression Of ProBNP And NTproBNP And SST2 In Sudden Death People Due To Coronary Atherosclerotic Heart Disease

Objective:To research the pro-brain natriuretic peptide(pro BNP)and Amimo-Terminal pro-brain natriuretic peptide(NTpro BNP)and Soluble Suppression of Tumorigenicity2(s ST2)in the differential diagnosic of sudden death patients due to coronary atherosclerotic heart disease.Methods:Totally 60 myocardial samples and 60 plasma samples were collected from the sudden cornary disease cases in the Department of Forenic Pathology,Shanxi Medical University during 2011-2016.All sample were divided into three group:sudden death of cornary atherosclerotic heart disease group,single coronary stenosis group and normal control group.pro BNP in myocardium were detected by Immunohistochemical staining and Western blotting.BNPm RNA in myocardium were detected by RT-q PCR.NTpro BNP and s ST2 in plasma were detected by ELISA.Result:Immunohistochemical staining showed that the positive staining proBNP appeared in the cornary atherosclerotic heart disease group and single coronary stenosis group.Western blotting showed that the pro BNP protein expression in the cornary atherosclerotic heart disease group and coronary stenosis group is higher than normal control group obviously.The relative quantitation of BNPm RNA in the cornary atherosclerotic heart disease group and single coronary stenosis group is higher than normal control group obviously(P<0.05).NTpro BNP and s ST2 in the cornary atherosclerotic heart disease group and single coronary stenosis group is higher than normal control group obviously(P<0.05).Among the three groups,the expression of NTpro BNP and s ST2 showed significantly differences through pairwise comparison(P<0.05).Conclusion:(1)pro BNP was increased in myocardial tissues during myocardial ischemia, and pro BNP could be used as a marker for myocardial ischemia and sudden coronary death.(2)NTpro BNP and s ST2 in plasma can help to identify the severity of coronary heart disease and whether it is sudden death due to coronary heart disease.To detect the expression of pro BNP and NTpro BNP and s ST2 may provide a new method for forensic pathological diagnosis of sudden death due to coronary heart disease.