The Association Between Relative Telomere Length Of Peripheral Blood Cell And Hypertension

Objective: The telomere plays an important role in various researches,such asanti-cancer drug， anti-aging drug and environmental health.Both genetic andenvironmental factors affect telomere length. The study aims to investigate the factorsinfluencing blood cell telomere length the association between telomere length andhypertension risk.Method: Community-dwelled subjects who underwent routine health examination inNingbo City, Zhejiang province, China were recruited and screened for hypertension.Subjests who were30years older were interviewed by questionnaires, and bloodsamples were collected. The questionnaires included general demographic data,lifestyle behaviors and eating habits. Physical examination included height, weight,waist circumference and blood pressure. Human genomic DNA from peripheral bloodcells were extracted by Blood genomic extraction kit. The Real-time PCR wasadopted to detect the relative telomere length (RTL) of peripheral blood cells.ANOVA and multiple linear regression analysis, t-test, logistic regression modelswere adopted to analyze the correlation between RTL, lifestyle behaviors andhypertension.Result:(1) The coefficient of variation of measurement of RTL by the quantitativePCRranged from0.11to0.20, suggesting that amplification effecicency wassatisfcated.(2) The RTL of2346specimen of peripheral blood cells ranged from0.2020to2.9718,(1.0733±0.4278). There was a negative correlation between RTL ofperipheral blood cells and age (p<0.01).(3) The average telomere length of367patients with hypertension was1.0245, and the668healthy subjects was1.12531.There was a statistically significant difference in RTL between hypertension andhealthy subjects (p=0.001). There was a gender differences in RTL in hypertensiongroup. Telomere shortening rate in men was faster than that in women in hypertensionpatient.(4) RTL was significantly correlated with the risk of EH. After adjusted forage, BMI, TG, exercise, vegetable intake and salt intake, shorter RTL wassignificantly associated with increased risk of EH.(5) After adjusted for age, RTL wasassociated with education level, exercise, physical activity and vegetable intake inhealth individuals (p<0.05). Multiple linear regressions show that shorter ageadjusted-RTL was associated with exercise and intake of more than200g per week offish, while age adjusted-RTL is positively correlation with higher intake than150g aday of vegetables.Conclusions:(1) The RT-PCR screening system is suitable for measuring the telomere length with a large number of samples.(2) The RTL had significant differencebetween hypertension and normal human group, for the RTL in hypertension group issignificantly shorter than normal group.(3) Education level, exercise, physicalactivity and vegetable intake had significant effects on RTL of community dwellinghealth individuals.(4) Age, BMI, TG and salt intake were important risk factorscontributing to the hypertension, exercise, vegetables intake and telomere length wereimportant protective factors contributing to the hypertension.