Chemluminescent Logical Operation Of Biological Reactions By Using Bionic Compartmental System

It is well known that cells are one of the most complex and sophisticated systems in nature.A large number of intracellular biochemical reactions occur all the time to maintain the life of the system functions,including signaling function and energy metabolism.Therefore,the various biological molecules involved in biochemical reactions are usually markers of biological health and disease status.For example,the concentration of glucose in the body is closely related to the normal maintenance of energy metabolism,and the abnormal elevation of glucose levels can lead to diabetes,which is a marker of diabetes.Most of the biomarker molecules involved in the biochemical reactions are carried out in the cascade of enzyme catalysis.Perform many kinds of biochemical reactions in the "crowded" cells mainly depend on the basic characteristics of the compartmentalized cell structure,that is,the cells in the various organelles can be regarded as different cell structure of the compartment.In recent years,scientists at home and abroad have constructed a variety of artificial cell model to explore the rule of cell life activities in response to the characteristics of compartmentalized cellular structure.In this paper,we take the property to construct bionic compartmentalized system,combined with the concept of Boolean logic gate,and high sensitive chemiluminescence as output signals,on the one hand through the logic output signal to detect and analyze biomarkers,on the other hand can make our understanding of the process of life of cells more simple.The contents of this paper include the following:Part one,Enzyme-based bio-cascade reactions and cell compartmentalized structures,the progress of the research on the bio-cascade and the compartmentalized structure is summarized.At the same time,the logic operation and chemiluminescence analysis method are introduced.The application of the biological field provides a theoretical basis for our study below.Part two,We first synthesize poly(ionic)liquid which constructed by multiple alternating hydrophilic and hydrophobic nanodomains,this feature offers facilitation for loading peroxyoxalate chemiluminescent sysytem to the part of hydrophobic,at the same time we have constructed a new type of nanocontainer with a hybrid of poly(ionic)liquid as the core and mesoporous silica as shell to solve the problem of dispersion after poly(ionic)liquid loading chemiluminescent sysytem in aqueous solution.In this paper,based on the chemiluminescence functional nanocontainer,we studied the peroxyoxalate and dye rubrene best encapsulation mole ratio,at the same time,the kinetics of the chemiluminescence functionalized nanocontainers was investigated.It was found that the chemiluminescence functionalized nanocontainers had high luminescent properties and could effectively maintain the luminous efficiency.Part three,liposome is a kind of artificial membrane,its bilayer structure is closest to the structure of the cell membrane,the preparation of compartmentalized structure of liposomes closer to the natural cell structure,and liposomes will interdigitate and fuse when under the phase transition temperature and the existence of ethanol conditions.Therefore,in this part we mainly use liposome to construct compartmentalized system,and combined with chemiluminescence functionalized nanocontainer what prepared in the first part,the nanocontainer first covered with a layer of liposome,and interaction with other liposomes thus construct a compartmentalized structure of a large number of nanocontainers with large liposomes.Through the hemolysin embedded into the liposome membrane formation of protein holes to promote the different compartment biomolecules transmembrane transfer and exchange.Part four,the concept of Boolean logic is introduced into our previous synthesis of bionic system.The oxidase enzymes are used as the input and chemiluminescent signal as the output.The chemiluminescent signal is used to analyze the biomarkers,and the chemiluminescent intensity evaluation of diagnosis and prevention of biomarker abnormalities caused by the disease.Because in the cell reaction complex diversity,in order to explore the process of life is more complex,so we expand the logic operation scale,a plurality of logic system are integrated in series,and by analyzing the responses in different logical states to simplify the understanding of the reactions that take place in the course of life.In this paper,the concept of logic gate is combined with the bionic compartmentalized system and chemiluminescent system for the first time,a simple analysis of the biomarkers and the cascade reactions of biomarkers under enzyme catalysis.We have successfully built several logical systems of AND,OR,INHIBIT,AND + OR,AND + INHIBIT,OR + INHIBIT and AND + OR + INHIBIT.From the analysis of biomarkers to the imitation of natural biological cascade,the introduction of our bionic and compartmentalized system and the introduction of the concept of logic will have a significant role in the diagnosis and prevention of disease and in the field of biomimics.