Study Of Tumor Markers Detection Based On Gold Nanocontainer

Part1In the process of the growth and breeding of cancer cells, the content of ATP in the cell will change obviously, on the other hand, in the death process of cell, the synthesis of ATP is restrained, the content of ATP in the cell will quickly reduce. Study the content of ATP in the cell is of great significance for our understanding of the metabolic state and lesion severity of cancer cells.In the experiment, we prepared the silver nanocube with the method of reduction trifluoroacetic acid and hollow, thin wall gold nanocontainer was synthesized by silver nanocube. The morphology of gold nanocontainer was preliminary characterized by transmission electron microscope (TEM) and ultraviolet spectrophotometer (UV). According to the TEM, the size of gold nanocontainer was in 30 to 40 nm; the gold nanoparticles was assembled into gold nanocontainers using DNA hybridization technology for DNA biosensor, and then we detect the amount of primers chain replaced down by ATP to obtain the concentration of ATP, and achieve the signal amplification using enzyme circulationamplification technology. In the experiment, under the best detection conditions, different concentrations of ATP was measured, ATP detection of linear range: 1.0×10-9～1.0×10-7mol/L, detection limit:8.8×10-10 mol/L(3σ). Experiments also measured the ATP in Hela extract, the determination results was 3.27×1O-6mol/L.Part 2We detected the thioalcohol with new electrochemical probe. The best test conditions for GSH:0.2 mol/L B-R buffer solution (pH 2.0) reaction in 20 min. Under this condition, target molecules GSH was measured, detection of linear range:1.0×10-10～1.0×10-9 mol/L, detection limit:1.0×10-10 mol/L(3σ, n=11). In addition, the new electrochemical probe response to metal sulfur protein (MT) and glutathione reductase (GR) was analysed by differential pulse voltammetry (DPV) and the current signal of new electrochemical probe on protein thiol (MT) and nonprotein thiol (GSH) was compared, the DPV analysis results showed that the current signal of the new electrochemical probe response to MT was obvious higher than that of GSH, namely the new electrochemical probe was more sensitive response to protein thiol.