Study On The Pharmacodynamics And Active Site Of Geerqiong Killing Protoscolexin Vitro

?Objectives?To elucidate the pharmacodynamics activity and active site of Tibetan medicine geerqiong and to explore the effect of the different extractions on the protoscolex in vitro.?Methods?Twenty-six compounds were isolated and purified by alcohol extraction,extraction,gel column chromatography,silica gel column chromatography and preparative liquid phase.The compounds were treated with protoscolex in different concentrations in vitro.Observe the mortality and structural changes of the protoscolex under light microscope.?Results?The mortality rate of protoscolex in contral group was 13.67±0.577 and ethanol extract group was 93.67±1.523(P<0.0000),ABZ group was 47.67±2.08(P<0.0000),the structure changes of protoscolex treated with ABZ and ethanol extract was significantly different.Three parts were obtained from the extraction of alcohol extract which named ethyl acetate part,n-butanol part and petroleum etherpart.We found that the mortality rate of protoscolex treated with ethyl acetate part was 93.67±0.577(P<0.0000).Fraction1,Fraction2,Fraction3,Fraction4,Fraction5 were obtained from the ethyl acetate site with gel column chromatography.The pharmaceutically active experiment found that the mortality rate of protoscolex treated with Fraction3 was 97.33±1.528(P<0.0000).(4)Fraction3.1,Fraction3.2,Fraction3.3,Fraction3.4,Fraction3.5,Fraction3.6,Fraction3.7,Fraction3.8 were obtained from Fraction3 with Silica gel column chromatography.The pharmaceutically active experiment found that the mortality rate of protoscolex treated with Fraction3.1 was 83.00±4.583(P<0.0000).(5)Fraction3.1.1,Fraction3.1.2,Fraction3.1.3,Fraction3.1.4,Fraction3.1.5,Fraction3.1.6,Fraction3.1.7 were obtained from Fraction3.1 with S liquid phase.The pharmaceutically active experiment found that the mortality rate of protoscolex treated with Fraction3.1 was 83.33±3.06(P<0.000).?Conclusions?Geerqiong has a stronger effect on protoscolex compared to albendazole in vitro and its active site is macromolecular non-polar compound.