The Effect Of Osthole On Oxidative Stress Response To Cerebral Ischemia-reperfusion In Mice Myocardium And Lung Tissue

After the attack of cerebral ischemia-reperfusion,some organ function would damage and even worse the occurrence of multiple organs damage and failure.One of the important injury mechanisms is to activate the oxidative stress system.Osthole?OST?belongs to natural coumarin compounds.The early experimental studies have found that OST has protective effects on multiple organs injury after cerebral ischemia reperfusion.Whether the responses of oxidative stress are involved in the protection of OST,it is the main content in this study.Objective: To observe the effect of OST on anti-oxidative indexes occurred in mice myocardium and lung tissue and analysis the protective mechanisms in cerebral ischemia-reperfusion mice model.Methods: A modified method Himori was used to temporarily block the bilateral common carotid arteries to prepare the model of cerebral ischemia-reperfusion injury in mice.The mice were randomly divided into 5groups,such as sham operated group?surgery,but without cerebral ischemia reperfusion?,control group?i.e.ischemia-reperfusion group?,Osthol in high dose group,middle dose group and low dose group,three groups were administrated wtih intraperitoneal?IP?injection OST 10,1,0.1mg�kg^-1,before30 min ischemia respectively,Sham group and control group were administrated with IP solvent respectively before ischemia.The study used biochemical to detect of heart and lung tissue of mice and plasma superoxide dismutase?superoxide dismutase,SOD?;catalase?catalase,CAT?;glutathione peroxidase?glutathioneperoxidase,GSH-PX?activity and malondialdehyde?malondialdehyde,MDA?content.Results:1 Myocardium of Cerebral ischemia reperfusion mice: Glutathioneperoxidase?GSH-PX?,superoxide dismutase?SOD?,catalase?CAT?activity,malondialdehyde?MDA?content in mice lung tissue,compared within the sham operated group,the control group GSH-PX,CAT and SOD in lung tissue activity decreased significantly,the content of MDA increased significantly?P<0.01?;cnidiadin control group cerebral ischemia and reperfusion in mice lung tissue GSH-PX,CAT,SOD activity was significantly increased,MDA content decreased significantly(OST 10mg�kg^-1 P <0.01,OSTl,0.1mg�kg^-1 P<0.05).2 Lung tissue of Cerebral ischemia reperfusion mice: Glutathione peroxidase?GSH-PX?,superoxide dismutase?SOD?,catalase?CAT?activity,malondialdehyde?MDA?content in myocardium,compared within the sham operated group,the control group GSH-PX ? CAT and SOD in Mice Myocardial activity decreased,but the MDA content increased significantly?P<0.01?;Osthole group cerebral ischemia and myocardial tissue perfusion in mice of GSH-PX,CAT,SOD activity was significantly increased,MDA content decreased significantly(OST10mg�kg^-1 P <0.01,OST l,0.1mg�kg^-1 P<0.05).3 Blood of Cerebral ischemia reperfusion mice: Glutathione peroxidase?GSH-PX?,superoxide dismutase?SOD?,catalase?CAT?activity,malondialdehyde?MDA?content in mice lung tissue,compared within the sham operated group,the Control group GSH-PX?SOD and CAT in the blood of mice decreased significantly,the content of MDA increased significantly?P<0.01?;the control group of osthole in cerebral ischemia reperfusion mice blood GSH-PX,CAT,SOD activity was significantly increased,MDA content decreased significantly(OST10,1mg�kg^-1 P <0.01).Conclusions: Cerebral ischemia-reperfusion operation decreases the activity of GSH-PX,SOD and CAT in the myocardium,lung tissue and blood,and increases the content of MDA.The QST alleviates the oxidative stress response to cerebral ischemia-reperfusion mice model.It hits the OST has a function to protect heart and lung tissue after cerebral ischemia-reperfusion.