Correlation Study Between IL-6 And Its Receptor And TCM Syndromes Of Ankylosing Spondylitis

Objective: The aim of this study was to investigate the association between IL-6,IL-6R and JAK1 and AS,objective To investigate the expression of IL-6,IL-6R,JAK1,PLT,CRP,ESR,C3,C4,IgG,IgM,IgA,AAG,CER,HPT,BASDAI and BASFI in patients with active ankylosing spondylitis,to explore the association between IL-6R rs4129267 locus and susceptibility to AS,and the association with TCM syndromes.Method: Collected in June 2015 ~ December 2016 from Gansu Provincial People's Hospital of Integrated Traditional Chinese and Western Medicine Department of rheumatology and outpatient and inpatient AS patients with 101 cases of experimental group,and selected from the hospital physical examination center of the normal health examination of 100 cases as the control group,and the experimental group of patients according to TCM syndrome differentiation,AS patients were recorded separately the age,sex,duration of disease,the clinical laboratory indicators(PLT,CRP,ESR,C3,C4,IgG,IgM,IgA,AAG,CER,HPT)were provided by the Gansu Provincial People's Hospital,using BASDAI,BASFI,refers to the distance and pillow wall from the evaluation of AS disease activity.Early morning fasting elbow venous blood to take serum and whole blood specimens.Serum levels of IL-6R and JAK1 in peripheral blood of patients with AS and healthy subjects were measured by ELISA.The expression of IL-6 mRNA in peripheral blood of patients with AS and healthy subjects was detected by RT-PCR.IL-6 mRNA,IL-6R,JAK1 and AS clinical laboratory indicators,TCM syndrome differentiation analysis.The genotype and allele frequency of IL-6R gene rs4129267 in peripheral blood of two groups were detected by PCR-RFLP,and IL-6R gene polymorphism and AS TCM syndromes were analyzed.Result: 1.IL-6mRNA?IL-6R?JAK1 and the relevance of AS:The levels of IL-6R and JAK1 in serum of AS patients were significantly higher than those of healthy people,the difference between the two groups was statistically significant(P <0.05),and the two were positively correlated.The expression of IL-6 m RNA in AS patients was higher than that in healthy subjects(P <0.05).2.IL-6mRNA,IL-6R,JAK1 and the course of disease,inflammation index(PLT,CRP,ESR,AAG,CER,HPT),immunoglobulin(IgA,IgG,IgM),complement(C3,C4),occipital wall distance,refers to the distance,BASDAI,BASFI correlation:There was a significant positive correlation between JAK1 and PLT,IgG,IgM,C3,C4,CRP,ESR,AAG,CER,occipital wall distance,finger distance,BASDAI and BASFI(P <0.01),and had a positive correlation with the course of disease(P <0.05),and IgA,HPT were not correlated(P> 0.05);there was a significant positive correlation between IL-6R and PLT,IgG,C3,C4,CRP,ESR,AAG,CER,finger distance,BASDAI and BASFI(P <0.01),and there was a positive correlation with occipital wall(P <0.05),there was no correlation between course of disease,IgA and HPT(P> 0.05);there was a positive correlation between IL-6 mRNA and pathology,PLT,CRP,ESR,occipital wall distance,finger distance,BASDAI and BASFI(P <0.05),there was no correlation between IgG,Ig M,IgA,C3,C4,HPT,AAG and CER(P> 0.05).3.The distribution of TCM syndromes in AS group:The retention of damp-cold syndrome(46cases,45.5%),the retention of stagnated blood syndrome(23cases,22.5%),the retention of damp-heat syndrome(18cases,17.8%),the deficiency of kidney-yang syndrome(9cases,8.9%),and the deficiency of liver-kidney syndrome(5cases,5.0%).Active AS is mainly about the retention of damp-cold syndrome.4.The association between TCM syndromes and IL-6mRNA,JAK1,IL-6R,course of disease,inflammation index(PLT,CRP,ESR,AAG,CER,HPT),immunoglobulin(IgA,IgG,IgM),complement(C3,C4),occipital wall distance,finger distance,BASDAI and BASFI 4.1IL-6 mRNA:The level of IL-6 mRNA in the retention of damp-cold syndrome was significantly higher than that in the deficiency of liver-kidney syndrome(P<0.05),the level of IL-6mRNA in the retention of stagnated blood syndrome was lower than that the deficiency of kidney-yang smyndrome(P<0.05),there was no significant difference in IL-6 mRNA level between the two groups(P> 0.05).4.2IL-6R,JAK1:The levels of serum JAK1 and IL-6R in the retention of damp-cold syndrome were significantly higher than those in other four types(P<0.05),the level of serum JAK1 and IL-6R in the retention of stagnated blood syndrome was higher than that in the deficiency of kidney-yang smyndrome and the deficiency of liver-kidney syndrome(P<0.05),the level of serum JAK1 in the retention of damp-heat syndrome was higher than that the deficiency of liver-kidney syndrome(P<0.05),the level of serum IL-6R in the retention of damp-heat syndrome was significantly higher than that in the deficiency of kidney-yang smyndrome and the deficiency of liver-kidney syndrome(P<0.05).There was no significant difference in JAK1 and IL-6R levels between the two groups(P> 0.05).4.3Course:Compared with the course of liver and kidney deficiency syndrome,the difference was statistically significant(P <0.05),there was no significant difference between the two groups(P> 0.05).4.4Inflammation index(PLT,CRP,ESR,AAG,CER,HPT):PLT:The expression level of PLT in the retention of damp-cold syndrome was significantly higher than that in other four types(P <0.05),there was significant difference between PLT and Kidney-yang deficiency syndrome and liver-kidney deficiency syndrome(P <0.05).CRP,ESR:The expression of ESR and CRP in the retention of damp-cold syndrome was higher than that of the other four types(P <0.01).AAG,CER,HPT:There was no significant difference in AAG,CER and HPT between TCM syndromes(P> 0.05).4.5Immunoglobulin(IgA,IgG,IgM):The expression of IgG and IgM in the retention of damp-cold syndrome was higher than that of the other four types(P <0.05),the expression of IgG,IgM in the retention of stagnated blood syndrome was compared with the deficiency of kidney-yang syndromeand the deficiency of liver-kidney syndrome,the difference was statistically significant(P <0.05).There was no significant difference in IgA between TCM syndromes(P> 0.05).4.6Complement(C3,C4):The expression of C3 in the retention of damp-cold syndrome was higher than that of the other four types(P <0.05),the expression of C3 in the retention of stagnated blood syndrome was compared with the deficiency of kidney-yang syndromeand the deficiency of liver-kidney syndrome,the difference was statistically significant(P <0.05).There was no significant difference among the TCM syndromes(P> 0.05).4.7Pillow wall:The difference between the two groups was statistically significant(P <0.05).4.8Refers to the distance:There was significant difference between the TCM syndromes(P <0.05),there is a significant difference between the retention of damp-cold syndrome and the deficiency of kidney-yang syndrome(P <0.01).4.9BASDAI,BASFI:The BASDAI and BASFI of the retention of damp-cold syndrome were significantly higher than those of the other four types(P <0.05),the difference was statistically significant(P <0.05).5.Analysis of IL-6R Gene Polymorphism:The allele and allele of IL-6R gene rs4129267 in AS patients were significantly different from those in healthy control group(P <0.05).The frequency of CT genotype in AS group was significantly different from that in control group(P <0.05).There was significant difference between the CT genotype of the retention of stagnated blood syndrome and the control group(P <0.05),there was no significant difference between the other genotype and the control group(P> 0.05).Conelusion 1.JAK1 are involved in the pathogenesis of AS.2.The retention of damp-cold syndromefor the active syndrome of the main type of AS.3.There was a correlation between the retention of damp-cold syndrome and PLT,CRP,ESR,IgG,IgM,C3,finger distance,BASDAI,BASFI,IL-6R,JAK1 and IL-6mRNA;the retention of stagnated blood syndrome was associated with PLT,IgG,IgM,C3,IL-6R,JAK1 and IL-6 mRNA;the retention of stagnated blood syndrome was a correlation between IL-6R,JAK1 and BASDAI.4.IL-6R gene rs4129267 gene locus and ankylosing spondylitis is closely related,CT genotype may be ankylosing spondylitis susceptibility gene.5.IL-6R gene rs4129267 locus CT genotype may be associated with the retention of stagnated blood syndromesyndrome.