| Objectives(1)To study the protective effect and mechanism of Mongolian medicine Bawei Chenxiang San on acute myocardial infarction(AMI)rats.(2)To compare the protective effect and mechanism of Bawei Chenxiang San with different animal heart on normal rats.Methods UPLC-Q-Exactive-MS technique is used to determine the endogenous metabolites in the plasma of sham operation group,AMI model control group and different dose of Bawei Chenxiang San groups,to determine the endogenous metabolites in the plasma of the blank control group and the group of Bawei Chenxiang San with different animals.The chromatographic conditions of the UPLC-Q-Exactive-MS technique are as follows: the column of Hypersil GOLD C18 column is used with a mobile phase of acetonitrile-0.1%formic acid solution using gradient elution at a flow rate of 0.2mlˇmin-1,and the column temperature is set at 40?.The mass spectrometry conditions are as follows: Spray current(ESI)(+/-)is 4.0/2.2 Kv,Capillary temp is 350?,Aux gas heater temp is 100?,Sheath gas flow rate is 40 arb,Aux gas flow rate is 2 arb,S-lens RF level is 70 V,Scan type is Full MS,Scan range is 50.0-750.0m/z,and Resolution is 70000.The data obtained from the experiment are analyzed by principal component analysis(PCA)and optimized potentials for liquid simulations-discriminant analysis(OPLS-DA).Results(1)The data model established by OPLS-DA is superior to PCA.The results show that the high and middle dose groups of Bawei Chenxiang San are well away from the AMI control group,being closed to the sham operation group.The low dose group is closed to the AMI control group,being away from the sham operation group.In the high dose,8 AMI related biomarkers are completely reversed,in the middle dose 5 AMI related biomarkers,and in the low dose 2 AMI related biomarkers.Among them,LysoPC(18:2(9Z,12Z)),lactic acid and creatine are all related to energy metabolism.LysoPC(18:2(9Z,12Z))and uric acid are related to inflammation and oxidative damage,and tryptophan,valine,nicotinamide and phenylalanine are related to amino acid metabolism.(2)The data model established by OPLS-DA is superior to PCA.The resultsshow that the group of Bawei Chenxiang San with yak heart or cattle heart is well away from the blank control group,and the effect of the prescription with yak heart is better than that of cattle heart.The group of the Bawei Chenxiang San with pig heart is closed to the blank control group,with partially overlapping.Bawei Chenxiang San with yak heart can regulate 9related biomarkers,which Bawei Chenxiang San with yellow cattle heart regulates 6 and Bawei Chenxiang San with pig heart regulates 3.In the regulated biomarkers,leucine,isoleucine,valine,tryptophan and phenylalanine are all related to amino acid metabolism.Linoleic acid,LysoPC(16:0),LysoPC(18:2(9Z,12Z))and LysoPC(18:1(9Z))are related to energy metabolism.LysoPC(16:0),LysoPC(18:2(9Z,12Z))and LysoPC(18:1(9Z))are also related to oxidative damage.Conclusions(1)The high and middle doses of Bawei Chenxiang San have protective and therapeutic effects on AMI model rats,and the effect of high dose on AMI model rats is the best.The low dose of the prescription is less effective on AMI model rats.The mechanism of pre-protection and treatment of Bawei Chenxiang San is mainly related to the regulation of energy metabolism and amino acid metabolism,anti-oxidative damage,and inhibition of inflammation.(2)Bawei Chenxiang San with different animal heart have a protective effect on normal rats.The effect of the prescription with yak heart is the best,the prescription with cattle heart is second,and the prescription with pig heart is the worst.The protective mechanism of Bawei Chenxiang San is mainly related to regulation of energy metabolism and amino acid metabolism and to reduction of oxidative damage. |
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