Effects Of Transplantation Of Embryonic Spinal Cells On Axon Regeneration After Delayed Repair Of Peripheral Nerve Injury

Peripheral nerve injury is not uncommon in clinical practice,about more than 1 million people suffered from peripheral nerve damage in the world each year,peripheral nerve injury often lead to the loss of physical movement and sensory function,even if timely diagnosis and clinical repair,Only 5%-10% of the total number of cases can fully restored distal limb function.The current study suggests that repair of peripheral nerve injury was unsuccessful is mainly due to: 1st.peripheral nerve regeneration is slowly,the roughly growth rate of the regeneration was 1mm / day.2.Peripheral nerve injury repair depends on the peripheral nerve injury micro-environment.And peripheral nerve injury micro-environment with the passage of time gradually adverse axonal regeneration.3.After prolonged denervation,the denervated muscles have been atrophic,fibrosis,etc.,these factors have a negative impact on axonal regeneration.Foreign literature reports and our previous studies have found that transplanting stem cells into injured nerves can improve the regenerative microenvironment after peripheral nerve injury.we hypothesized that in the chronic degeneration of the injured peripheral nerve distal,by transplanting embryonic spinal neurons,can also improve the postoperative repair is not conducive to axonal growth into the microenvironment,thereby promoting the proximal axon growth.To this end,this study will explore the delayed repair of peripheral nerve injury in the local transplantation after implantation of embryonic spinal cord suspension on the proximal axonal regeneration.?Objective? To explore the effect and mechanism of transplanted rat embryonic spinal cord cells on proximal axon regeneration in delayed repair of peripheral nerve injury.To further explore the delayed repair of peripheral nerve injury,improve the damage to repair microenvironment and improve the effect of delayed repair to provide experimental basis and new ideas.?method?(1)30Male SD rats were randomly divided into two groups according to the random number method:(1)After the injury,the rats were injected with injection group(control group).(2)Cell group(experimental group).The left tibial nerve was transected,and the proximal and distal ends were ligated into the submucosal and the left gastrocnemius muscle was dominated by the animal model.(2)2 months after the first operation,according to the group and the number of experimental animals: "pre-modified left tibial nerve distal-fresh cut off the common peroneal nerve proximal end-toe anastomosis",and in surgery After the success of the nerve anastomosis,3ul(3ul)was injected into the culture medium(control group)3ul or embryo spinal cord suspension(experimental group)at the distal 5mm of the anastomosis.(3)After 3 months of animal feeding,electrophysiological examination was performed to detect the wet weight of bilateral gastrocnemius muscle,the repair of nerve segment and the gastrocnemius cross-sectional staining and exercise end plate staining.The differences of wet weight and muscle staining between the experimental group and the control group were compared with those of the experimental group and the control group.The survival and differentiation of the transplanted cells were compared.?result? "Spontaneous nerve proximal-tibial nerve distal anastomosis +injection of embryonic spinal cord cells or culture medium" 2 months later,the experimental group to repair the segmental regeneration of myelinated axon count,nerve cross-sectional area and gastrocnemius wet(P <0.05).The results were statistically significant(P <0.05).The results were statistically significant(P <0.05).?conclusion?In the 3 months after the degeneration of the tibial nerve injury,the "proximal peroneal nerve-distal tibial nerve" cross anastomosis repair,in the anastomosis after the distal nerve injection of embryonic spinal cord cell suspension can promote the anastomosis after the axillary approach End of the regeneration,dominate the muscles and promote the distal nerve after the re-control of muscle function recovery.