Experimental Study On The Repair Of Spinal Cord Injury In Rats With Delayed Implantation Of Autologous Nerve

Objective: To investigate the pathological and fluoro gold(FG)retrograde tracing changes of autogenous nerve delayed transplantation on spinal cord injured in rats.Methods:The animals were divided into two groups,with modified Allen impact method to establish model of spinal cord injury.After 4 weeks,the transplantation group using autologous sural nerve graft to repair spinal cord injury period and non-transplantation group was only exposed incision without treatment.In the 4,6 and 8 weeks after operation,observation of regeneration of the injured spinal cord in the light microscope was performed.the retrograde tracing of FG was performed to discover the recovery of the axial plasma transport simultaneously.Results:In the non transplantation group,spinal cord represented degeneration,cystic cavity and cicatrix was found.In the experimental group,most of peripheral nerve survived,and was fused with the spinal tissue with time lapse after transplantation.Compared with the non-transplantation group,there were significant differences(P<0.01).FG positive labeled neurons were sparse in 4 weeks.At 6 weeks after transplantation,FG positive cells were gradually increased,and the FG positive cells were concentrated in 8 weeks after transplantation.Compared with the control group,there were significant differences(P<0.01).Conclusion:After spinal cord injury,autologous nerve graft is repaired and survived well and promote the regeneration of spine and the recovery of spinal cord injury segment shaft pulp transportation function.