Expression And Meaning Of MiR-195 And IKBKB In Placenta Of Pre-eclampsia

Objective :To explore the expression and functions of mi R-195 and IKBKB in maternal placenta of preeclampsia.Methods :The maternal placenta and clinical data of 30 patients with preeclampsia and 30 cases of normal pregnant women were be collected between July 2015 and April 2016 in the Second Hospital of SHANXI Medical University.Real Time quantitative PCR detects the expression of mi R-195 in placenta,bioinformatics analysis between mi R-195 and IKBKB extracted from mi RWalk2.0,the expression of IKBKB which is a target protein of mi R-195 in placenta was detected by immunohistochemical and western-blot method,SPSS 17.0 is used for statistical analysis.Results :Age(t=1.86)and BMI(t=0.92)in two group had no significant differences(P>0.05),while the gestational weeks [(32.8±0.77)vs(39.02±0.69),t =6.24,P(27)0.01 ],systolic blood pressure [(180.9±9.2)vs(118.6±3.27),t =7.38,P(27)0.01 ],diastolic blood pressure[(118.7±6.02)vs(69.2±2.18),t =8.82,P(27)0.01 ]and birth weight[(1737±201.3)vs(2870±193.2),t =3.84,P(27)0.01 ]in two groups were obviously different(P<0.01).The real time-PCR reasults of mi R-195 was significantly decreased in severe preeclampsia placenta,which is(0.36±0.20)times as much as healthy pregnant woman.Western-blot detection results of IKBKB in preeclampsia pregnant women was decreased compared with that in healthy pregnant women[(0.08±0.01)vs(0.13±0.01),t=13.94,P<0.001)].Correlation analysiss show there is no correlation between mi R-195 and IKBKB in this experiment(r=0.1227,P=0.5503).Conclusion :Mi R-195 is downregulated in preeclamptic pregnant women while IKBKB is downregulated in preeclampsia placenta.The down-regulation of mi R-195 and the down-regulation of IKBKB may regulate the apoptosis of trophoblastic cells,which may participate in the physiopathologic mechanism of preeclampsia.