The Role Of IL-33 In The Inflammatory Response Of Allergic Rhinitis With PM2.5 Exposure

Objective:In this paper,we investigated whether PM2.5 exposure could aggravate the inflammatory reaction of allergic rhinitis,and the role of IL-33 in the allergic rhinitis,and the possible pathway.Methods:1.Acquisition PM2.5,physical and chemical analysis and preparation of contaminated samples.2.Establishment of a mouse model of allergic rhinitis,behavioral scoring model.3.After successful modeling,PM2.5 intervention was used to establish the model of allergic rhinitis in mice exposed to PM2.5;4.Treatment of allergic rhinitis in mice exposed to PM2.5 by anti IL-33antibody;Three groups of mice peripheral blood smear were taken to count eosinophils,ELISA detection of IgE and IL-33 levels;ELISA detection of IgE and IL-33levels;morphological changes of nasal mucosa were observed;nasal mucosa was detected by Western blot,the expression of NF-?B.Results:1.Contaminated sample of PM2.5 particles were successfully prepared;2.The mouse model of allergic rhinitis was successfully modeled with typical symptoms;3.Peripheral blood smear results:The eosinophil count in AR model group was lower than that inPM2.5 intervention group,there was statistical difference between two groups(P<0.05);The eosinophil count of anti IL-33 treatment group was lower than that of PM2.5 intervention group,there was statistical difference between two groups(P<0.05);4.ELISA results:The serum IgE and IL-33 levels in the AR model group were significantly lower than those in the PM2.5 intervention group,there was statistical difference between two groups(P<0.05);The levels of serum IgE and IL-33 in anti IL-33 treatment group were significantly lower than those in PM2.5 intervention group,there was statistical difference between two groups(P<0.05);5.Results of nasal mucosa HE staining:AR model group:The epithelial cells of nasal mucosa were disordered,the cilia were shed,and the infiltration of inflammatory cells such as eosinophils and neutrophils.PM2.5 intervention group:The structure of nasal mucosa epithelial cells was incomplete,the cilia were shed,and the infiltration of inflammatory cells such as eosinophils and neutrophils in the submucosa was obvious,tissue edema,glandular hyperplasia.Anti IL-33 treatment group:The epithelial cells of nasal mucosa were arranged in order,the structure of cilia was complete and mild inflammatory cell infiltration.Western blot results:The NF-?B in nasal mucosa of AR model group was lower than that of PM2.5 intervention group,there was statistical difference between two groups(P<0.05);The level of NF-?B in nasal mucosa of anti IL-33 group was lower than that of PM2.5 group,there was statistical difference between two groups(P<0.05).Conclusion:1.PM2.5 has small size,large area,high activity and complex components,and is easy to be attached to heavy metals,pathogenic microorganisms,polycyclic aromatic hydrocarbons and their derivatives.2.PM2.5 can aggravate allergic rhinitis mucosa inflammatory reaction.3.Elevated IL-33 levels in peripheral blood of AR mice exposed to PM2.5.4.Anti IL-33 antibody can alleviate mucosal inflammatory response in mice with allergic rhinitis.5.IL-33 may play a role in the NF-?B pathway.