Role Of Cell Pyroptosis In Neonatal Necrotizing Enterocolitis

Objective To explore the role and potential possible mechanism of cell pyroptosis in neonatal necrotizing enterocolitis.Methods One hundred 1-day-old Sprague-Dawley rats were randomly divided into 2 groups,control group and NEC group(n = 50).The neonatal rats in control group were left with their mothers,and not submitted to stress and all the pups in NEC group were stressed with gavage feeding,hypoxia and cold stress to induce the model of NEC.The weights of pups were measured in 3 consecutive days at the same time.The genenal condition of rats were observed,and the incidence rate of NEC and the mortality were calculated in the experiment.All rats were killed on the fourth day via decapitation and 2 cm sections of the proxima l intestines of ileocecal junction were harvested for histopathologic evaluation.The mRNA expression of NLRP3,IL-1?,IL-18 were tested by Quantitative real-time PCR.The expression and activation level of caspase-1 were detected by Western blotting.The levels of IL-1? and IL-18 in intestine tissues were evaluated by ELISA.All statistical analyses were performed with SPSS software version 22.0.The data were presented as the means ± standard deviation.Normal distribution homogeneity of variance data us ing the independent sample t test;normal distribution but variance not neat data using the Mann-Whitney test.P<0.05 was considered to be statistically significant.Results Compared with the control group,the weight was decreased and the intestinal injury was obviously aggravated in the NEC group.The NEC group exhibited higher gene expression of NLRP3,IL-1? and IL-18(P<0.05)and higher expression of IL-1?,IL-18 proteins in intestine tissues compared with control group.Moreover,the activated caspase-1 protein only expressed in NEC group.Conclusion A stable necrotizing enterocolitis neonatal rat model was established.The necrotizing enterocolitis neonatal rats had obviously intestinal injury.Cell pyrotosis is involved in the pathogenes is of necrotizing enterocolitis,and the mechanism may be related with the role of IL-1? and IL-18.