Regulatory Effects And Mechanism Of Atractylenolide ? On Aquaporin3,4 Of Rats With Slow Transit Constipation

Objective This study took slow transit constipation as animal model to study he changes of AQP3 and AQP4 under the condition of diseases,and further explore the mechanism of atractylenolide ? of STC.Methods We selected forty healthy Wistar rats which were 7-8 weeks old and weight(170-220)g.They were randomly assigned to the control(n=10)and the STC model group(n=30).There was no significant difference in the weight between the two groups.According to the literature,Rhein were used to establish the slow transit constipation mode.After the establishment of the model,the rats were randomly divided into model group(n=10),atractylenolide ? group(n=10),mosapride group(n=10).Each group was given the corresponding measures to intervene,the stool weight(wet weight)and fecal character were recorded daily,the carbon powder propulsion rats in the instestine were measured and the expression of AQP3,AQP4 in rat colon were detected by the immunohistochemistry and RT-PCR.Results(1)Fecal character in model group was more dry and hard(Bristol classes:1-2grade)than in normal group(Bristol classes: 4-5grade);The wet weight of stools in24 hours has no significant difference compare the normal group with the model group before the establishment of the model(P > 0.05),the wet weight of stools in 24 hours has significant difference compare the normal group with the model group after the establishment of the model(P < 0.05);Intestinal transit rate in the normal group was87.24±1.34%,and the model group was 63.58±3.76%,the model group was lower than the normal group(P < 0.05).(2)By comparing with the normal group,the model group of the intestinal transit rate declined significantly,the wet weight of stools in 24 hours decreased significantly(P < 0.05);By comparing with the model group,the atractylenolide ? group and the mosapride group of the intestinal transit rate risen significantly,the wet weight of stools in 24 hours increased significantly(P < 0.05);The different of the intestinal transit rate and the wet weight of stools in 24 hours between atractylenolide ? group and mosapride group has no significant difference(P > 0.05).(3)Immunohistochemistry revealed: Under light microscope,AQP3 and AQP4 were mainly expressed in the colonic epithelial cells,goblet cells and pit cell.Immunohistochemical picture in brown or tan is positive expression.By comparing with the normal group,the colonic epithelial cells and goblet cells of the model group were stained more strongly;By comparing with the model group,atractylenolide ? group,mosapride group of colonic epithelial cells and goblet cells were negative or weakly positive staining.The different of immunohistochemistry between atractylenolide ? group and mosapride group has no significant difference(P > 0.05).(4)RT-PCR:By comparing with the normal group,the optical density value of AQP3 ? AQP4 in model group increased significantly,has significant statistical significance(P<0.05);By comparing with the model group,the optical density value of AQP3 ?AQP4 in atractylenolide ? group and mosapride group decreased significantly,has significant statistical significance(P < 0.05);The different of the optical density value of AQP3 ? AQP4 between atractylenolide ? group and mosapride group has no significant difference(P > 0.05).Conclusion(1)Compared with the normal group,the optical density value of AQP3?AQP4 in model group was increased,which indicated that over expression of AQP3 and AQP4 may be one of the pathogenesis of STC.(2)The atractylenolide ? can regulate the expression of AQP3?AQP4 in proximal colon of the STC in rats,which indicated that atractylenolide ? can balance the absorption and secretion of water,increase intestinal transmission power,improve the efficacy of gastrointestinal dysfunction.