The Role And Mechanism Of SOX4 Gene In Chronic Myelogenous Leukemia

Objective To explore the role of SOX4 in chronic myelogenous leukemia(CML)and its mechanism in regulating chronic myelogenous leukemia through interference,in addition,to explore the possibility of SOX4 as a CML target.Methods K562 cells of CML-BP were cultured and si RNA-SOX4 plasmid p LVE-1680,the empty plasmid p LVE were instantaneous transfected into K562 cells,then we investigated the SOX4 m RNA and protein level after transfection.p53,bax,c-myc,cyclin D1 and bcl-2 gene expression level were detected by q RT-PCR,while the expression of Foxo3 a,C/EBP? were used RT-PCR.Cell proliferation was assayed with CCK8 kit.Gene chip detects differences between the interference group and the control group.Results RT-PCR and Western blot showed that si RNA-SOX4-K562 cells were successfully constructed.The expression level of C/EBP?,bax,Foxo3 a,cyclin D1 m RNA in si RNASOX4-K562 group were 0.1609±0.0095,1.2410±0.0602,1.1450±0.0461,1.7240±0.0657 respectively,which were significantly higher than that of in K562 and K562-empty group(p<0.01,p<0.05,p<0.05,p<0.05),while the expression level of c-myc,bcl-2,p53 in si RNASOX4-K562 group were 0.9171±0.009534,0.6507±0.009021,0.4952±0.02205 respectively,which were significantly lower than that of in K562 and K562-empty group(p<0.01,p<0.01,p<0.01).We found that cell proliferation level in si RNASOX4-K562 group was significantly lower than that in K562 and K562-empty proup(p<0.05).Limited to 2 times differences in gene,gene chip screening results indicated tnat interference group had 4 higher gengs than the control group and seven relatively lower than the control group.Two groups differentially expressed genes of Pathyway enrichment function analyzed that the most concentrated Pathyway were platelet activation,leukocyte transendothelial migration and focal adhesion.Conclusion SOX4 was as an oncogene in CML by affecting cell proliferation,differentiation and apoptosis,and interference of SOX4 could inhibit K562 cell growth and promote cell apoptosis.Gene chip screening results showed that SOX4 may reduced RAP1 A,and up-regulated SNX2,ZNF486,AX747507 gene to participate the pathogenesis of CML.These suggested that SOX4 may play important roles in the pathogenesis of CML,and regulate the expression of SOX4 may become the potential point for myelogenous leukemia treatment.