The Expression And Relevant Analysis Of Macrophage Marker CD68 And CD163 In Marginal Liver Tissue And Serum Of Patients With Hepatic Alveolar Echinococcosis

Objective:CD68 and CD163 were different functional status markers of macrophage,recognized as an essential cell in other parasitic infection.This research was aimed to explore the expression of CD68 and CD163 in the tissue and peripheral blood of the patients with hepatic alveolar echinococcosis,and the role of macrophages in development of hepatic alveolar echinococcosis.Methods:Expressions of CD68 and CD163 in marginal zone of lesions and normal liver tissue from patients with hepatic alveolar echinococcosis underwent surgery were detected by immunohistochemistry,and concentrations of s CD68 and s CD163(soluble form of CD68 and CD163)in serum were measured by ELISA.The relationships of CD68 and CD163 with patients' characteristics were assessed by the Mann-Whitney test.Associations between expressions of CD68 and CD163 in tissue and serum were evaluated by linear regression analysis.Results:The positive cell rate of both CD68 and CD163 were statistical higher in marginal zone than those in normal liver tissue(60% vs.10%,85% vs.15%).However,there were no difference between expression of CD68 and CD163 in marginal zone(P>0.05).The average concentration(ng/ml)of both s CD68 and s CD163 in patients group were also statistical higher than those in health control(11.36±3.46 vs.2.57±2.72,209.73±186.40 vs.86.34±135.80),and concentration of s CD163 was significantly higher than that of s CD68 in patients group(P<0.05).Moreover,level of CD68 and CD163 in both tissues and serum was positively correlated(R2=0.896,P<0.01;R2=0.491,P<0.05).Conclusions:The level of CD68 and CD163 were significantly increased in marginal zone of HAE lesions,and s CD68 and s CD163 were also increased in HAE patients' serum.Macrophages may be play roles in the pathogenesis of hepatic alveolar echinococcosis.