| Objectives:The aims of this study were to investigate the correlation between the levels of inflammatory adipocyte cytokines including interlukin-6(IL-6),interlukin-1?(IL-1?),monocyte chemotactic protein-1(MCP-1)and adiponectin(ADP)with the prevalence and severity of coronary artery disease(CHD)in patients undergone coronary angiograph(CAG).Methods:In this cross-sectional study,we consecutively enrolled 240 inpatients with preliminary diagnosis of CHD undergone CAG in our hospital.Then were divided into CHD group(n=159,57.89±9.48 years old)and Non-CHD group(n=81,56.33±7.95 years old).The serum levels of IL-6,IL-1?,MCP-1 and ADP were measured by enzyme-linked immunosorbent assay(ELISA),and serum protein expression levels were measured by Western Blotting.In addition,the gene expression levels of them were determined by real-time polymerase chain reaction(RT-PCR).Results:1 Clinical features: compared with the Non-CHD group,the CHD group had more male patients[73.6%(117/159)vs 49.4%(40/81),P<0.001],the prevalence of type 2 diabetes mellitus(T2DM)and current smoking were significantly higher in CHD group than those in Non-CHD group[27.7%(44/159)vs 12.5%(10/81);54.7%(87/159)vs 21%(17/81),all P<0.05].2 The levels of inflammatory factors:2.1 Detection of serum levels by ELISA: the serum levels of IL-6,IL-1?,MCP-1 were significantly higher than those in Non-CHD group,the results were 27.3(21.5,37.5)pg/mL vs 21.0(8.8,33.5)pg/mL;13.8(6.4,61.1)pg/mL vs 9.5(4.7,22.2)pg/mL;282.0(123.5,395.1)pg/mL vs76.6(39.5,175.4)pg/mL,the differences were statistically significant(all P<0.05),while ADP level was conversely,the serum level of ADP in CHD group was significant lower than in Non-CHD group,the result was 2.4(1.8,3.1)ng/mL vs 5.0(3.2,6.3)ng/mL(P<0.05).2.2 Detection of serum protein expression levels by Western Blotting: the serum protein expression levels of IL-6,IL-1?,MCP-1 were significantly higher than those in the Non-CHD group,the results were 1.3(1.0,1.7)vs 0.9(0.6,1.2);1.2(0.90,1.5)vs 0.9(0.8,1.1);1.2(1.0,1.6)vs 0.9(0.7,1.5),the differences were statistically significant(all P < 0.05),while the ptotein expression level of ADP in CHD group was significant lower than in Non-CHD group,the result was 0.9(0.8,1.2)vs 1.2(1.1,1.5).2.3 Detection of the gene expression levels by RT-PCR: the blood mRNA expression levels of IL-6,IL-1?,MCP-1 were significantly higher than those in the Non-CHD group,the results were 8.1(2.7,11.4)vs 1.3(0.4,3.1);5.0(2.4,12.1)vs 1.2(0.4,2.3);11.4(6.7,28.6)vs 1.1(0.3,2.4),the differences were statistically significant(all P<0.05),while the mRNA expression level of ADP in CHD group was significant lower than in Non-CHD group,the result was 0.1(0.0,0.4)vs 0.4(0.1,2.5).3 Spearman correlation analysis:3.1 The serum levels of IL-6,MCP-1 were positive correlation with Gensini Score(Rs=0.182;0.378),the differences were statistically significant(all P < 0.05);the serum concentration of ADP and Gensini Score had significant negative correlation(Rs =-0.526)(P < 0.05).3.2 The serum protein expression levels of IL-6,IL-1 beta and MCP-1 were positively correlated with the Gensini Score(Rs =0.423;0.377;0.405),the differences were statistically significant(all P < 0.05);while correlation between the serum protein expression level of ADP and Gensini Score was significant negative(Rs=-0.381)(P < 0.05).3.3 The blood mRNA expression levels of IL-6,IL-1 beta,MCP-1 were positively correlated with Gensini Score(Rs=0.431;0.496;0.694)the differences were statistically significant(all P < 0.05);the blood mRNA expression level of ADP was significantly negatively correlated with the Gensini Score(Rs =-0.314)(P < 0.05).3.4 The correlations of inflammatory factors with clinical characteristicsThe serum level of IL-6 was positively correlated with current smoking,LVESD,NLR,left ventricular end-diastolic dimension(LVEDD)(Rs= 0.173,0.172,0.163,0.145),the differences were statistically significant(all P < 0.05);the serum level IL-1 beta was negatively correlated with sex,while positively correlated with T2 DM,SBP(Rs=-0.157,0.159,0.301),the differences were statistically significant(all P < 0.05);the serum level of MCP-1 was positively with WBC and NLR(Rs=0.149,0.175),there were statistically significant(all P < 0.05);the serum level of ADP was negatively correlated with current smoking,systolic blood pressure(SBP),left ventricular end-systolic diameter(LVESD),brain natriuretic peptide(BNP),white blood cell count(WBC),neutrophil to lymphocyte ratio(NLR),low density lipoprotein(LDL),ad mission blood glucose(Rs=-0.146,-0.226,-0.203,-0.359,-0.328,-0.223,-0.159,-0.15),the differences were statistically significant(all P < 0.05).4 ROC curve analysis showed that the areas under the curve of IL-6,IL-1 beta,MCP-1,ADP in diagnosis of patients with CHD were: 0.669(95%CI: 0.585,0.753),0.598(95%CI: 0.512,0.685),0.805(95%CI: 0.746,0.864),0.884(95%CI: 0.841,0.928)(all P < 0.05);the optimal cut-off points were 17.37pg/mL,10.18 pg/mL,190.06 pg/Ml,2.54ng/mL respectively;the sensitivities were 94.8%,58.7%,67.6% and 98.8%,and the specificities were 43.8%,58.9%,89.2% and 53.7%,respectively.Conclusions:1 Serum levels and serum protein expression levels and blood mRNA gene expression levels of IL-6,IL-1 beta,MCP-1 in CHD group were significantly higher than those in Non-CHD group,while serum level of ADP,while the corresponding expression levels of ADP in CHD group was significantly lower than those in Non-CHD group.2 The serum levels of IL-6,MCP-1,ADP were not affected by the anthropometry,including age,sex and body mass index(BMI).3 Gensini Score was an indicator of quantitative assessment of coronary artery stenosis,which was positively correlated with the serum levels of IL-6,MCP-1 and negatively correlated with the serum level of ADP,indicating that the serum levels of ADP,IL-6 and MCP-1 can reflect the severity of coronary artery stenosis,which can be used as biological markers to assessment of the severity of coronary artery stenosis.Inflammatory adipocyte cytokines,such as IL-6,IL-1 beta,MCP-1 and ADP were significantly correlated with the prevalence of CHD and the coronary artery stenosis,may be used as important biomarkers for the clinical diagnosis and evaluation of CHD. |
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