Study On Brain Functional Mechanism Of "Dispersing Liver And Regulating Spirit" Acupuncture Therapy On Post-traumatic Stress Disorder Based On The Technology Of FNIRs

Objective: Detection of post-traumatic stress disorder(PTSD)in rats model related to the brain blood oxygen concentration change,based on neural vascular coupling mechanism,then evaluation related brain neuron activity,reveals the characterization of spatial distribution in abnormal activated brain regions and response model;At the same time explore “Dispering Liver and Regulating Spiit” god stitch intervention of PTSD rats cortex and functional reconstruction of space distribution of activated brain regions,And comparative analysis and the effect of western medicine paroxetine difference,“Dispering Liver and Regulating Spiit”god was revealed stitch intervention brain function mechanism of PTSD,as acupuncture intervention provide basis for the clinical application and mechanism of the effect of PTSD.Method: 60 healthy male SD rats(180 + 20 g),Using the method of computer random number were randomly divided into blank group,model group,fetching,the paroxetine group and acupuncture group.Except the blank group each group of rats in shock claustrophobic copy PTSD animal model,the model copy a total of seven days.At the start of the building,filling and paroxetine group rats take paroxetine hydrochloride solution(0.42 mg/m L-1,10 m L · kg 1)treatment;Acupuncture group selection "GV20" and "PC6","HT7","LR3 four holes,“Dispering Liver and Regulating Spiit”acupuncture treatment;Fetching groups take the same as the treatment group of fetching method is fixed.Building after the end of each treatment group after treatment for 5 consecutive days collect and record the using f NIRs system between groups in the rat brain tissue related oxy-Hb,Deoxy-Hb and changes in the concentrations of the Total-Hb assessing brain funct Results:1 Each channel oxy-Hb,Deoxy-Hb and Total-Hb concentration time sequence comparison(1)Foxy-Hb concentration within three minutes of time sequence change results: compared with the blank group,model group,fetching fluctuation,appear many sharp peaks,change is obvious;Compared with capturing group,paroxetine group,acupuncture group is with flat,sharp peaks decreases,obvious variations in flat.Statistical results show that the oxy-Hb time sequence,model group,the fetching group and blank group had significant difference(P < 0.01;P < 0.01);Model group and fetching group there was no significant difference(P > 0.05);Compared with capturing group,paroxetine group,the acupuncture groups had significant difference(P < 0.05;P< 0.05);And paroxetine group and acupuncture group there was no significant difference(P > 0.05).Deoxy-Hb concentration in 3 minutes time series changes: compared with the blank group,model group,fetching fluctuation,appear many sharp peaks,change is obvious;Compared with capturing group,paroxetine group,acupuncture group is with flat,sharp peaks decreases,obvious variations in flat.Oxy-Hb,Deoxy-Hb time sequence change presents tradeoffs,and a negative correlation.Statistical results show that the Hb time sequence,model group,the fetching group and blank group had significant difference(P < 0.01;P < 0.01);Model group and fetching group there was no significant difference(P > 0.05);Compared with capturing group,paroxetine group,the acupuncture groups had significant difference(P < 0.05;P < 0.05);And paroxetine group and acupuncture group there was no significant difference(P > 0.05).Groups of 10 rats channel Oxy-Hb,Deoxy-Hb and Total-Hb concentration change histogram analysis results: Compared with the blank group,model group Oxy-Hb,Deoxy-Hb and Total-Hb concentrations have obvious changes,Compared to the model group,the density showed a obvious change with the significant difference(P< 0.01),fetching Oxy-Hb,Deoxy-Hb and Total-Hb concentration has no obvious change,compared with the model group,the concentrations of Oxy-Hb,Deoxy-Hb and Total-Hb were not significantly changed(P> 0.05);Compared with holding group,paroxetine group,acupuncture group Oxy-Hb,Deoxy-Hb and Total-Hb concentrations were significantly change,statistically significant difference(P < 0.05).(2)Channel foxy t value changes in brain regions Oxy-Hb concentration distributionModel group compared with the blank group,people with PTSD foxy-Hb concentration change obvious areas,mainly in the prefrontal cortex,hippocampus,amygdala,anterior cingulate activation is obvious,at the same time in the inferior frontal gyrus,frontal gyrus area in the inhibitory state.Paroxetine group and fetching Oxy-Hb concentration is more noticeable than brain regions is given priority to with the hippocampus and prefrontal cortex.Than acupuncture group and fetching Oxy-Hb concentration are the obvious changes of brain regions in the hippocampus and the prefrontal and parahippocampal gyrus.2 Hemodynamic(plan)According to the stereotaxic atlas rats and f NIRS hemodynamic plane graph has established thefollowing 10 approximate area.The analysis of the two sample t test results:There is a two-way change the cerebral cortex.Compared with the blank group,model group rats cortex Oxy-Hb concentration change obvious brain regions in the prefrontal cortex,hippocampus and temporal back to give priority to;Compared with capturing group,paroxetine group rats cortex Oxy-Hb concentration change obviously brain regions in the prefrontal cortex,hippocampus and the amygdala is given priority to;Compared with capturing group,acupuncture group rats cortex Oxy-Hb concentration change obvious brain regions before cingulate gyrus,frontal lobe,hippocampus and the amygdala is given priority to;After compare the screening finally with the most obvious change is the prefrontal cortex,hippocampus,amygdala as the main area for research.(2)Prefrontal Oxy-Hb,Deoxy-Hb and Total-Hb concentration change resultsCompared with blank group,model group Oxy-Hb,Deoxy-Hb and Total-Hb concentration has obvious changes,statistically significant difference(P < 0.01),compared with model group,fetching Oxy-Hb,Deoxy-Hb and Total-Hb concentration has no obvious change,Compared with the model group,the concentrations of Oxy-Hb,Deoxy-Hb and Total-Hb were not significantly changed(P> 0.05),paroxetine group,acupuncture group Oxy-Hb,Deoxy-Hb and Total-Hb concentration had significant changes,statistically significant difference(P < 0.05).(3)The hippocampus Oxy-Hb,Deoxy-Hb and Total-Hb concentration change resultsCompared with blank group,model group Oxy-Hb,Deoxy-Hb and Total-Hb concentration has obvious changes,statistically significant difference(P < 0.01),compared with model group,fetching Oxy-Hb,Deoxy-Hb and Total-Hb concentration has no obvious change,Compared with the model group,the concentrations of Oxy-Hb,Deoxy-Hb and Total-Hb were not significantly changed(P> 0.05),paroxetine group,acupuncture group Oxy-Hb,Deoxy-Hb and Total-Hb concentration has a significant change,statistically significant difference(P < 0.05),than with the paroxetine group,acupuncture group Oxy-Hb,Deoxy-Hb and Total-Hb concentration has obvious changes,statistically significant difference(P < 0.05).(4)The amygdala Oxy-Hb,Deoxy-Hb and Total-Hb concentration change resultsCompared with blank group,model group Oxy-Hb,Deoxy-Hb and Total-Hb concentration has obvious changes,statistically significant difference(P < 0.01),compared with model group,fetching Oxy-Hb,Deoxy-Hb and Total-Hb concentration has no obvious change,Compared with the model group,the concentrations of Oxy-Hb,Deoxy-Hb and Total-Hb were not significantly changed(P> 0.05),paroxetine group,acupuncture group Oxy-Hb,Deoxy-Hb and Total-Hb concentration has obvious changes,statistically significant difference(P < 0.05).Conclusion:1 PTSD in the rat model of cerebral cortex Oxy-Hb,Deoxy-Hb and Total-Hb concentration,indicate the brain neuron activity,cell energy metabolism and hemodynamics disorders,the characterization of the abnormal activated brain regions of cerebral cortex and function abnormalities,mainly the prefrontal cortex,hippocampus and the amygdala,which may be one of the central mechanism of PTSD.2 “Dispering Liver and Regulating Spiit”god stitch can obviously improve the abnormal cortex of rats oxygenated hemoglobin,shows that acupuncture treatment effectively;“Dispering Liver and Regulating Spiit” god stitch intervention PTSD exists differences between local cerebral cortex with the model group,may be“Dispering Liver and Regulating Spiit” god stitch for PTSD improvement important central mechanism,and the corresponding brain nerve functional remodeling is the main reason.“Dispering Liver and Regulating Spiit” god stitch intervention PTSD main central mechanism and prefrontal cortex,hippocampus,amygdala brain regions associated plasticity.In conclusion,“Dispering Liver and Regulating Spiit”gods stitch can improve PTSD cerebral oxygenation hemoglobin and related brain nerve function restore,play a good treatment effect.