Mechanism Of Hippocampus In Rats With Post Traumatic Stress Disorder

Post traumatic stress disorder(PTSD)is a delayed and long-lasting mental disorder caused by abnormally threatening or catastrophic traumatic events.The symptoms of PTSD are very complicated,and the symptoms of different PTSD patients are not identical,resulting in insufficient accuracy of conventional treatment methods and unstable treatment.The occurrence of PTSD is associated with impaired brain function in the hippocampus.The function of the hippocampus has sub-region specificity.The functional diversity of different subregions of the hippocampus may be closely related to the complexity of PTSD disorders.However,there is currently no relevant research.In this project,the UWT(under water trauma)model rats with more phenotypic diversity and human PTSD patients' complexity were studied.The hippocampus brain regions were studied by behavioral,electrophysiological and molecular biology techniques to discovery the pathogenic mechanisms.The main findings of this project are as follows:1.UWT model is successfully builtThe results of water O labyrinth,open field experiment and light dark box experiment showed that the anxiety level of UWT rats increased;the results of sucrose preference test showed that the level of depression in UWT rats increased;the results of contextual fear conditioning test showed that UWT rats had impaired fear extinction ability.It can be seen that UWT rats have impaired fear-regression ability and exhibit anxiety-like and depression-like behavior,indicating that the UWT model was successfully constructed.2.Different pathways in different subregions of the hippocampus of UWT rats(the dorsal and ventral Schaffer collateral pathways as well as the dorsal and ventral medial anterior perforating pathways)have normal synaptic transmission capacity and LTD is impaired.The results of in vitro brain field potential experiments showed that the inputoutput curve and paired-pulse ratio of different pathways in different hippocampus of UWT rats were normal,that is,the basic synaptic transmission ability was normal,and LTD was damaged.3.The expression levels of NR2 A,NR2B,GluA1 and GluA2 in synaptic bodies of different subregions of hippocampus(back and ventral CA1 and dorsal and ventral DG)were normal,while ?CaMK? activity increased.In order to study the molecular mechanism,we performed Western blotting and found that the expression of NR2 A,NR2B,GluA1 and GluA2 in different sub-regions of hippocampus of UWT rats was normal,while the activity of ?CaMK? increased.In summary,we found that the changes of NMDA receptor AMPA receptor and ?CaMK? in different pathways of different subregions of hippocampus and hippocampus in UWT rats were similar.However,these similar changes can cause damage to different functions of UWT rats.This project study will help to further understand the complexity of PTSD disorders and provide a theoretical basis for the study of PTSD precision treatment methods.