Dynamic Expression And Significance Of Related Non-coding RNA In Plasma Of Patients With Viral Hepatitis B

Objectives To explore the dynamic expression of four kinds of the related non-coding RNA(miR-122,miR-155,lnc RNA MALAT1,lnc RNA H19),which is in plasma of patients with viral hepatitis B(HBV),and its association with inflammation,cell cycle factors.Analysis the significance of four kinds of the related non-coding RNA in viral hepatitis B.Methods Subjects: Divided into healthy control group of 101 people,hepatitis B group of 86 people(points on time were divided into 0w,1w,2w,3w,4w group).Field investigation: medical records obtained general situation,such as research object of gender,age,smoking and drinking habits,liver function tests results,etc;at the same time,blood samples were collected from objectives stored in-80? fridge.Laboratory testing: we used real-time fluorescence quantitative PCR method to detect the expression of miR-122,miR-155,lnc RNA MALAT1,lnc RNA H19;enzyme linked immunosorbent assay(ELISA)was used to detect inflammatory cytokines(IL-6,TNF-alpha)and cell cycle factors(p53,Cyclin D1)protein content.Statistical analysis: mean±standard error(x ±SE)was used to indicate measurement data;dynamic analysis of each index at different time points by using repeated measures ANOVA;Inter group analysis of healthy control group and hepatitis B group of each index using single factor analysis of variance;correlation analysis using Pearson correlation analysis.Results First of all,general factors analysis: gender,age,educational level,smoking and drinking had no significant difference in patients with hepatitis B and healthy controls.Thus,in all parameters between the two groups the expression difference is may not affected by the above factors,with a balanced comparability.Secondly,through repeated measurement of variance analysis showed that these four kinds of non-coding RNA of miR-122,miR-155,lnc RNA MALAT1,lnc RNA H19 in plasma of patients with hepatitis B were all high expression(P<0.05);With the clinical treatment and remission,miR-122 increased first and then decreased,and the expression of miR-155,lnc RNA MALAT1 and lnc RNA H19 decreased.Thirdly,through the correlation analysis the dynamic expression of the four kinds of non-coding RNA and the level of ALT,AST,found that show a positive correlation between them(P<0.01),so that expression of the four kinds of non-coding RNA correlate closely to liver injury induced by viral hepatitis B.Fourth,the result of analysis of the relationship between four kinds of non-coding RNA and inflammatory cytokines(IL-6,TNF-?),show that is a positive correlation(P<0.01),especially miR-155,lnc RNA MALAT1,lnc RNA H19.Fifth,analyzed the relationship between the four kinds of non-coding RNA and cell cycle related factors(p53,Cyclin D1),tried to find out the role of these four non coding RNA in viral hepatitis B.It found that four kinds of non-coding RNA were positively correlated with Cyclin D1;but miR-155,lnc RNA H19 were negatively correlated with p53(P<0.01).These four kinds of non-coding RNA are closely related to cell cycle,and the interaction mode is different.Last but not least,the correlation analysis between miRNA and lnc RNA was carried out.The results showed that miR-155 was more closely related to the two lnc RNA than miR-122.Conclusions In summary,four kinds of non-coding RNA(miR-122,miR-155,lnc RNA,lnc RNA,MALAT1)were found to be abnormal expression in the plasma of patients with hepatitis B virus,which were associated with liver injury caused by hepatitis B virus,and involved in the inflammatory response and cell cycle regulation of hepatitis B virus.It provides a new perspective for the monitoring and treatment of liver injury in hepatitis B virus.Besides,miR-155 and lnc RNA MALAT1 and lnc RNA H19 may participate in the occurrence and development of hepatitis B virus.