Impact Of Accupuncture On The Expression Of PI3K/AKT Signaling Pathway Within Lung Tissue Of Asthmatic Rats

Objectives To study the role of PI3K/AKT(Phosphoinositide 3-kinase and AKT)signaling pathway in the pathogenesis of bronchial asthma in rats,and observe the impact of acupuncture on PI3K/AKT signaling pathway in bronchial asthma rat lung tissue,and explore the molecular mechanism that acupuncture treat asthma.Methods In the experiment,forty SPF male Sprague-Dawley(SD)rats were randomly divided into the control group(C),the asthmatic model group(M),the acupuncture group(A)and the blocking group(B)with 10 rats in each group.The rats asthmatic models were exploited,in which rats were sensitized with OVA and Al(OH)3,and repeatedly exposed to aerosolized ovalbumin.The rats in model group were disposed according to the above method.The rats in acupuncture group and blocking group were respectively treated by acupuncture and aerosol inhalation blocking agent LY294002 every time before aerosol challenge.The control group were sentisized and challenged by saline instead of OVA without therapies.All the interventions including acupuncture,aerosol inhalation OVA and LY294002 were implemented once every two days and in total 14 tines.firstly,In 24 hours after last challenge,the rats were anesthetized with 10% chloral hydrate followed by tracheal and esophageal intubation to estimate the change of respiratory function in rats according to determine the pulmonary elastic resistance and lung compliance.Then HE staining was employed to observe the lung tissue morphologic changes to determine weather the asthma models were established or not.The expressions of PI3 K,AKT and PAKT protein were assayed by immunohistochemical staining.Finally the level of PI3 K,AKT and P-AKT in lung homogenate Was measured by westem blot.Results 1 The results of respiratory function show:Compared with the control group,the pulmonary elastic resistance was increased(P<0.05)with lung compliance reducing(P<0.05)in asthmatic model group;Compared with the asthmatic model group,the pulmonary elastic resistance was reduced(P<0.05)with lung compliance increasing(P<0.05)in the acupuncture group and blocking group.2 HE-stained lung tissues of rats show:In control group,there was no inflammatory cells around the bronchus and the blood vessels,and bronchial structure was integral without mucous plug in lumen;A variety of inflammatory cells were infiltrated around the bronchus and the blood vessels with bronchial smooth muscle spasm and the lumen stenosis in asthmatic model group;Compared with the asthmatic model group,these changes in the acupuncture group and blocking group were obviously alleviated.3 The results of immunohistochemistry:Immunohistochemical staining indicated that expressions of PI3 K,AKT and P-AKT protein of lung tissues in the asthmatic model group were higher than that of other groups(P<0.05),and there was no significant difference between the control group and treated group(P>0.05).4 The results of Western Blot:The content of PI3 K,AKT and P-AKT protein of lung homogenate in the asthmatic model group were higher than that of other groups(P<0.05),and there was no significant difference between the control group and treated group(P>0.05).Conclusions 1 The P13K/AKT signal transduction pathway was activated in the asthmatic model rats,and asthma attack may be regulated by P13K/AKT signal tranduction pathway.2 Acupuncture can obviously reduce pulmonary elastic resistance of asthma rat,reduce inflammatory cells infiltration in airway and and alleviate airway lumen stenosis.3 Acupuncture can reduce the expression of PI3 K,AKT and P-AKT protein in the lung tissue,which can inhibit the activation of PI3K/AKT signal pathway similar to LY294002.