The Effect Of Exogenous H₂S On Health Rat Skin Fibroblast Proliferation

Objective: The influence of exogenous H2S on Rat skin fibroblast proliferation invitro.Method: when rat skin fibroblast in the logarithmic growth phase, make it into single cell suspension, the concentration of it is1×106/ml. Culture1mlcell suspension in12well culture plate48hours. Change the cell suspensioninto the no fetal bovine serum DMEM medium，culture it24hours until cellsynchronization. The cells were divided into four control groups，the group Bis cell suspension, the group C is Physiological saline，group NaHS(N1,N2,N3)and group PPG(P1,P2,P3),each group have three holes,200L DMEM medium with appropriate concentration drug were added respectively to three holes. After24hours, we get single cell suspension, then count and adjust the concentration. Detection suspension by flow cytometry. The measurement data expression by the mean±standard deviation (x±s), one-way ANOVA, α=0.05.Result: There is no Significant difference amount group NaHS（N1，N2，N3）,group B and group C（45.49±1.60、48.10±2.09、44.67±0.89%、46.12±1.02%：P>0.05）; There is no Significant difference amount group PPG（P1，P2，P3）,group B and group C（47.43±0.60、47.79±1.26、40.22±1.30%、46.12±1.02%：P>0.05）; There is no Significant difference amount group NaHS, group PPG,group B and group C（P>0.05）.Conclusion: Exogenous H2S has no Proliferation effect on rat skin fibroblastsin vitro.