| Objectives Detecting the protein expression of PD-1 and PD-L1 and its gene statu of KRAS in NSCLC paraffin-embedded specimen.Investigating the relevance of the PD-1/PDL1 protein expression with K-RAS gene mutations and its relationship with NSCLC clinical pathological features.Methods Collecting 64 NSCLC and 20 benign lung lesions tissues embedded in paraffin,the clinical data of all cases was complete,and then the protein expression of PD-1 and PD-L1 were detected by immunohistochemical staining,the K-RAS mutation were examined by real-time fluorescent quantitative PCR.Results 1 The positive rate of PD-1/PD-L1 protein expression were respectively 54.7%(35/64)and 28.1%(18/64)in 64 cases of NSCLC,whereas they were all negative in benign lung lesion,the positive rate was 0%(0/64),the difference was statistically significant(P<0.01).2 In 64 cases of NSCLC,the positive rate of PD-1 protein expression was 52.4%(22/42)in male group and 59.1%(13/22)in female group,there was no significant difference in the gender group by statistical analysis(P=0.403);the positive rate was 50.0%(14/28)in <60 years old and 54.7%(21/36)in ?60 years old,there was no significant difference in the age group by statistical analysis(P=0.506);the positive rate was 55.6%(20/36)in patients with smoking and 53.6%(15/28)in patients without smoking,there was no significant difference in the smoking group by statistical analysis(P=0.464);the positive rate was 50.0%(18/36)in lung adenocarcinoma and 60.7%(17/28)in lung squamous carcinoma,there was no significant difference between the tumor histologic type group by statistical analysis(P=0.274);the positive rate was 56.3%(9/16)in moderate and poor differentiated lung adenocarcinoma and 45.0%(9/20)in well differentiated one,there was no significant difference in the tumor differentiation situtation of lung adenocarcinoma by statistical analysis(P=0.502);the positive rate was 60.0%(15/25)in moderate and poor differentiated lung squamous cell carcinoma and 66.7%(2/3)in well differentiated one,there was no significant difference in the differentiation situtation of lung squamous cell carcinoma by statistical analysis(P=1.000);the positive rate was 52.0%(13/25)in patients with lymph node metastasis and 56.4%(22/39)in patients without lymph node metastasis,there was no significant difference in the lymph node metastasis situation group by statistical analysis(P=0.464);the positive rate was 47.7%(21/44)in ?-? stage and 70.0%(14/20)in ?-? stage,there was no significant difference in the TNM stage group by statistical analysis(P=0.097).The positive rate of PD-L1 protein expression was 21.4%(9/42)in male group and 40.9%(9/22)in female group,there was no significant difference in the gender group by statistical analysis(P=0.089);the positive rate was 28.6%(8/28)in <60 years old and 27.8%(10/36)in ?60 years old,there was no significant difference in the age group by statistical analysis(P=0.944);the positive rate was 19.4%(7/36)in patients with smoking and 39.3%(11/28)in patients without smoking,there was no significant difference in the smoking situation group by statistical analysis(P=0.071);the positive rate was 44.4%(16/36)in lung adenocarcinoma and 7.1%(2/28)in lung squamous carcinoma,there was significant difference in the tumor histologic type by statistical analysis(P=0.002);the positive rate was 62.5%(10/16)in moderate or poor differentiated lung adenocarcinoma and 30.0%(6/20)in well differentiated one,there was no significant difference between the tumor differentiation situtation by statistical analysis(P=0.051);the positive rate was 8.0%(2/25)in moderate or poor differentiated lung squamous cell carcinoma and 0.0%(0/3)in well differentiated one,there was no significant difference in the tumor differentiation situtation by statistical analysis(P=1.000);the positive rate was 24.0%(6/25)in patients with lymph node metastasis and 30.8%(12/39)in patients without lymph node metastasis,there was no significant difference in the lymph node metastasis situation group by statistical analysis(P=0.385);the positive rate was 31.8%(14/44)in ?-? stage and 20.0%(4/20)in ?-? stage,there was no significant difference in the TNM stage group by statistical analysis(P=0.330).3 In 64 cases of NSCLC,the mutation rate of K-RAS gene was 12.5%(8/64),the mutation rate of K-RAS was respectively 7.1%(3/42)in male group or 22.7%(5/22)in female group,there was no significant difference in the gender group by statistical analysis(P=0.073);the mutation rate was respectively 17.9%(5/28)in <60 years old or 8.3%(3/36)in ?60 years old,there was no significant difference between the age group by statistical analysis(P=0.285);the mutation rate was 11.1%(4/36)in patients with smoking or 14.3%(4/28)in patients without smoking,there was no significant difference in the smoking situation group by statistical analysis(P=0.721);the mutation rate was 22.2%(8/36)in lung adenocarcinoma and 0.0%(0/28)in lung squamous carcinoma,there was significant difference in the tumor histologic type by statistical analysis(P=0.008);the mutation rate was 37.5%(6/16)in moderate or poor differentiated lung adenocarcinoma and 10.0%(2/20)in well differentiated one,there was no significant difference in the tumor differentiation situtation by statistical analysis(P=0.103);the mutation rate was 8.0%(2/25)in patients with lymph node metastasis and 15.4%(6/39)in patients without lymph node metastasis,there was no significant difference in the lymph node metastasis situation group by statistical analysis(P=0.383);the mutation rate was 9.1%(4/44)in ?-? stage and 20.0%(4/20)in ?-? stage,there was no significant difference in the TNM stage group by statistical analysis(P=0.244).In 36 cases of lung adenocarcinoma,the mutation rate of KRAS gene was 22.2%(8/36),the mutation rate of K-RAS was 18.8%(3/16)in male group or 25.0%(5/20)in female group,there was no significant difference between the gender group by statistical analysis(P=0.486);the mutation rate was 31.3%(5/16)in <60 years old and 15.0%(3/20)in ?60 years old,there was no significant difference in the age group by statistical analysis(P=0.422);the mutation rate was 30.8%(4/13)in patients with smoking and 17.4%(4/23)in patients without smoking,there was no significant difference in the smoking situation group by statistical analysis(P=0.422);the mutation rate was 16.7%(2/12)in patients with lymph node metastasis and 25.0%(6/24)in patients without lymph node metastasis,there was no significant difference between the lymph node metastasis situation group by statistical analysis(P=0.691);the mutation rate was 15.4%(4/26)in ?-? stage and 40.0%(4/10)in ?-? stage,there was no significant difference in the TNM stage group by statistical analysis(P=0.179).4 In 64 cases of NSCLC,the positive rate of PD-1 protein expression was 62.5%(5/8)in K-RAS mutation type and 53.6%(30/56)in K-RAS wild type,there was no relationship between K-RAS gene mutation status and PD-1 protein expression intensity(r=0.059,P>0.05);the positive rate of PD-L1 protein expression was 37.5%(3/8)in K-RAS mutation type and 26.8%(15/56)in K-RAS wild type,there was no relationship between K-RAS gene mutation status and PD-L1 protein expression intensity(r=0.079,P>0.05).In 36 cases of lung adenocarcinoma,the positive rate of PD-1 protein expression was 62.5%(5/8)in K-RAS mutation type and 46.4%(13/28)in K-RAS wild type,there was no relationship between K-RAS gene mutation status and PD-1 protein expression intensity(r=0.134,P>0.05);the positive rate of PD-L1 protein expression was 37.5%(3/8)in K-RAS mutation type and 46.4%(13/28)in K-RAS wild type,there was no relationship between K-RAS gene mutation status and PD-L1 protein expression intensity(r=-0.075,P>0.05)Conclusions 1 The positive rate of PD-1 and PD-L1 protein expression was higher in NSCLC than benign lung lesions.2 There was no relationship among PD-1 protein expression intensity with its gender,age,smoking condition,histologic type,differentiation,lymph node metastasis and TNM stage.The PD-L1 protein expression intensity was associated with the histological type of NSCLC.The positive rate of PD-L1 expression was higher in lung adeocacinoma than lung squamous carcinoma,but there was no relationship among PD-L1 protein expression intensity with its gender,age,smoking condition,differentiation,lymph node metastasis and TNM stage.3 The mutation of KRAS gene was associated with NSCLC histologic type,the mutation rate was higher in lung adenocarcinoma than in lung squamous cell carcinoma,but there was no relevance among K-RAS gene mutation with its gender,age,smoking condition,differentiation,lymph node metastasis and TNM stage.4 There was no relationship between PD1/PD-L1 protein expression with K-RAS gene mutation. |
PDF Full Text Request