| Objectives To explore the effects of sodium ferulate (SF) on rats with acute mesenteric venous thrombosis (MVT) in rats, and to explore its possible mechanism.Methods 96 male SD rats were randomly divided into three groups, 32rats in each group.Group A was the control group with normal saline, B group was treated with low molecular weight heparin group, C group was treated with sodium ferulate+low molecular heparin group. MVT model was made by ligate mesenteric vein and arcuate veins of rat 6cm long small intestine. A group was injected with normal saline 2ml/kg after operation,once every 12 hours, up to 72 hours after operation. Group B was given subcutaneous injection of low molecular weight heparin (300IU/kg) at 12 hours after operation, up to 72 hours after operation. Subcutaneous injection of low molecular weight heparin (300IU/kg)in the C group was performed every 12 hours after injection, and sodium fermlate 30mg/kg was injected into the tail vein every time for 24 hours, up to 72 hours after operation. The rats in each group were taken 8 hours, 24 hours, 48 hours and 72 hours, and then taken out of the 8 cases to check the abdominal cavity, and collected blood samples of inferior vena cava and long 3cm lesions of intestinal tissue.The levels of thromboxane B2 (TXB2) and plasma D-Dimer in different groups were detected. The 10% tissue homogenate was prepared from the diseased intestinal tissue and the content of malondialdehyde (MDA)and the activity of superoxide dismutase (SOD) were determined. By observing the intestinal tissue slices, according to the damage of intestinal tissue. All data were expressed as mean + standard deviation (LSD-t), and the same time points were compared with single factor analysis of variance, and 22 were compared with the least significant difference test.In the case of uneven variance, the nonparametric Kruskal-Wallis test was used to test H,and 22 were compared with Neymine test. Test level alpha =0.05.Results All of the 96 rats were successfully completed the modeling operation, and were completely awake within 2h after operation, the state of mind was good, the movement was free and the reaction was sensitive. The rats in each group were well tolerated, and the 12h began to eat after the operation, and remained alive until 72h. The results of pathological examination showed that the intestinal damage in each group decreased gradually with time. The injury score of A group was the highest, and the intestinal injury was the most serious. In group C, the repair of intestinal injury was the fastest, and in 72 hours after operation, the intestinal mucosa had been restored to normal structure. At different time periods, there were significant differences between the three groups in intestinal pathological damage (P < 0.05). Compared with the A group, the scores of the B group at the same time points were reduced, the difference was significant between 24 hours and 72 hours, while the scores of the C group at the four time points were significantly lower than those in the A group, the difference was statistically significant.The scores of C group at each time point after operation were lower than those in group B,the difference was significant between 24 hours and 72 hours (P < 0.05). The serum TXB2 level of the three groups decreased gradually with the passage of time, and the difference was statistically significant (P < 0.05). Compared with A group, B group at the time points of serum levels of TXB2 were lower, differences in 24 hours, 48 hours and 72 hours were statistically significant, while in C group, the serum levels of TXB2 in the four time periods were significantly lower than that of A group, a statistically significant difference(P < 0.05). The TXB2 level in C group was significantly lower than that in group B (P <0.05). The plasma D-Dimer level of the three groups decreased with the prolongation of time, and there was a significant difference between the two groups (P < 0.05). The plasma D-Dimer level of C group was significantly lower than that of A group and B group at each time point. The plasma D-Dimer level of B group was lower than that of A group at each time point, the difference was statistically significant (P < 0.05). Three groups of rat intestinal tissue MDA levels with time prolongation decreased, the level of MDA of intestinal tissue at different time points in C group was significantly lower than that of A group and B group, the level of MDA in intestinal tissue at different time points in B group than in the A group, after 24 hours, 48 hours and 72 hours significantly (P < 0.05). Three groups of intestinal tissue of rats SOD levels increased with time, B group and C group at each time point, the level of SOD was significantly higher than that of A group, the level of SOD of intestinal tissue at different time points in C group than in the B group, after 8 hours, 48 hours, 72 hours significantly (P < 0.05).Conclusions 1 The sodium ferulate and low molecular weight heparin have combined action on the treatment of mesenteric venous thrombosis. 2 The protective effect of sodium ferulate on intestinal tract is related to the inhibition of excessive metabolism of arachidonic acid four, the improvement of intestinal microcirculation, and the improvement of the ability of scavenging free radicals. |
