A Study On The Impact Of ME1 On Tumour Growth And Underlying Molecular Mechenism

Tumor is one of the main diseases in China and kidney cancer,a common tumor in urinary system,is one of them.At present,early diagnosis and resection surgery are still the treatment of kidney cancer,but there are many patients with kidney cancer has entered middle-late when diagnosed,and some cases of tumor have had cell metastasis,so in order to improve the diagnosis and enhance the treatment effect of kidney cancer,kidney cancer should be done more research to find more effective diagnosis and treatment.Malic enzyme(ME)is a metabolic enzyme which catalyzes the conversion of malic acid into pyruvate and produces NADPH or NADH.Malic enzyme in mammalian cells have three main kinds of subtypes,according to its orientation in the intracellular named respectively as malic enzyme 1(ME1)and malic enzyme 2(ME3)and malic enzyme 3(ME3).This paper focuses on the role and molecular mechanism of ME1 in renal cell carcinoma.The cell lines RC1 and RC2,which were mainly used for renal cell carcinoma,were infected by virus packaging and infected cell lines of kidney cancer,which led to the expression of ME1 in RC1 and RC2 cell lines,and screened the expression stable clone cell lines.The effects of ME1 on proliferation of renal cell carcinoma were detected by MTT assay and subcutaneous tumor of nude mice.The effects of ME1 on renal cell migration were detected by wound healing assay and transwell assay.The effects of ME1 on the cycle of renal cell carcinoma were detected by flow cytometry.To rearch on the effects of ME1 on metabolism,the glucose consumption and the content of lactic acid and ATP in the cells were detected.The results showed that the expression ME1 can inhibit the metabolism,proliferation and metastasis of kidney cancer cells,and also cause the cell cycle arrest to stop the cell resting on G1.The protein expression levels and mRNA transcription levels of the cell migration and invasion were detected in stable cloning cell line of ME1 overexpression,and the expression of the cell cycle protein was detected by western blot.The experimental results showed that after ME1 expression,the expression of vimetin in RC1 cells decreased,and the expression of E-cadherin protein increased.The expression of p21 and p27 also increased.And the mRNA levels involved in cell migration including E-cadherin,vimetin,ZEB1 and Twsit,did not have significantly difference.ME1 in renal cell carcinoma can inhibit the activity of transcription factor NF-?b,and gene expression of downstream of transcription factor NF-?b can be further explored.Cell cycle is a functional indicator of tumor detection,and the specific mechanism for studying ME1 to cause cell cycle arrest is the focus of the next step.