Analysis Of MiR-155 In Micro Vesicles From Patients With Coronary Artery Heart Disease

[Background]Coronary artery heart disease(CHD)can be defined as a syndrome caused by coronary atherosclerosis in a variety of pathogenic factors,which is characteristic of vascular stenosis or occlusion,myocardial ischemia and anoxia and myocardial infarction or remodeling.Recent studies have illustrated that dysfunction of endothelial progenitor cells,endothelial cells and smooth muscle cells may play a leading role in vascular disease.In the meantime,micro vesicles is a breed of micro substance released by various stimulation.According to some studies,these substance play an indispensable role in the regulation of miRNA signal transduction and functional expression.It is generally recognized that MVs behaves the main carriers of mi RNAs.MicroRNAs can regulate gene expression at the post transcriptional level via inhibiting protein translation or mRNA degradation.Meanwhile,they can control cell cycle by specific signal transduction,playing a significant pathophysiological role in cell differentiation,proliferation and apoptosis,hormone secretion and other biological processes.Many of mi RNAs are associated with CHD.As an inflammatory related miRNA,miR-155 has been proved to be involved in the development of vascular endothelial injury.[Object]To find out the expression level of miR-155 in the patients,and to explore the correlation between miR-155 and coronary heart disease.[Method]Based on the inclusion and exclusion criteria of this study,the peripheral blood samples of 92 hospitalized patients in Department of Cardiology of the Affiliated Hospital of Guangzhou Medical University were collected strictly from January 2014 to December 2016.Within these cases,4 groups were classified.The first group,referred to as Group A,has 24 cases of CHD.The second group,referred to as Group B,has 21 cases of type 2 diabetes mellitus.The third group,referred to as Group C,has 22 cases of coronary heart disease and type 2 diabetes mellitus.And the last group,referred to as negative control group,has 25 cases without history of coronary heart disease,type 2 diabetes mellitus and impaired glucose tolerance.By performing qRT-PCR and collecting various clinical date with the above-mentioned samples,they were conducted statistical analysis.Hereby certified,all data of this study were analyzed by SPSS v16.0.The statistical methods is single factor analysis of variance.[Results]There was no statistically significance in gender,age,TBIL,DBIL,IBIL,Cr,UA,BNP and LVEF among the four groups.CHOL in patients with coronary heart disease group had an increased trend compared with the negative group,there was significant difference between the two groups(P<0.05).LDL-C was significantly higher than in the negative control group(P<0.01).The TG,OGTT 2h,HbA1 C and left atrial diameter in the diabetic group were higher than in the negative control group(P<0.05),and the HDL-C was lower than that in the negative control group(P<0.05).The systolic pressure in the diabetic group were significantly lower than that in the negative control group(P<0.05).The TG,OGTT 2h,HbA1 C and left atrial diameter in patients with coronary heart disease combined with diabetes had an increased trend compared with the negative control group(P<0.05),and HDL-C was lower than in the negative control group(P<0.05).There was a significant difference in the expression of miR-155 in micro vesicles of patients with the CHD group and T2 DM group(P<0.01).And it had a significant difference between the CHD combined with T2 DM group and the negative control group(P<0.05).There was no statistically significance among the CHD group,T2 DM group and the CHD combined with T2 DM group(P>0.05).[Conclusion]1.The expression level of miR-155 in plasma membrane vesicles in patients with CHD,T2 DM and CHD combined with T2 DM was significantly higher than common people.2.There was no difference in the expression of miR-155 in plasma membrane vesicles in patients with CHD,T2 DM and CHD combined with T2 DM.